Effects of estradiol administration in vivo on testosterone production in two populations of rat Leydig cells.
Effects of estradiol administration in vivo on testosterone production in two populations of rat Leydig cells.
复制标题
体内雌二醇给药对两个大鼠 Leydig 细胞群睾酮产生的影响。
DOI：
10.1016/s0006-291x(82)80145-9
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
Abney,TO
中科院分区：
文献类型：
--
作者：
Keel,BA;Abney,TO
The effects ofin vivoadministration of estradiol on isolated rat testicular Leydig cells were investigated. Adult intact rats were injected s.c. with 50 μg/ 100 g B.W. of 17β-estradiol or vehicle twice daily for 2 days. Twelve hours after the last injection, collagenase dispersed interstitial cells were obtained and Leydig cells were subsequently isolated on metrizamide gradients. Two distinct peaks of specific125I-hCG binding corresponding to population I and II Leydig cells were observed. The hCG binding profile was unaltered as a result of estradiol treatment. Although twice as much testosterone was produced in population II, the responsiveness of the two populations to hCG or dbcAMPin vitrowas identical (11-to 13-fold increase). Estradiol administrationin vivoresulted in a 33–48% decrease in basal and stimulated testosterone production in both populations. These data indicate for the first time that both population I and II Leydig cells are sensitive to the direct inhibitory effects of estrogens on testosterone production. This inhibitory effect was not associated with an alteration in hCG binding capacity in either population. Therefore, we conclude that no functional difference exists between the two populations of Leydig cells with respect to the action of estrogens.
