ENSURING ANIMAL WELFARE WHILE MEETING SCIENTIFIC AIMS USING A MURINE PNEUMONIA MODEL OF SEPTIC SHOCK
ENSURING ANIMAL WELFARE WHILE MEETING SCIENTIFIC AIMS USING A MURINE PNEUMONIA MODEL OF SEPTIC SHOCK
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DOI：
10.1097/shk.0b013e3182939831
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发表时间：
2013-06-01
期刊：
影响因子：
3.1
通讯作者：
Chin-Dusting, Jaye P. F.
中科院分区：
文献类型：
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作者：
Huet, Olivier;Ramsey, Debbie;Chin-Dusting, Jaye P. F.
在动物模型中，故意将死亡作为终点在道德上是不可接受的。然而，在脓毒性休克的研究中，死亡仍然被认为是唯一相关的终点。我们将8个人类终点定义为4个严重程度阶段（从健康到死亡），并用于设计临床相关评分工具，称为“小鼠败血症临床评估评分”（M-CASS）。使用M-CASS来实现疾病严重程度评估的一致性方法。这允许在实施安乐死后对疾病进行道德和客观的评估，而不是加重痛苦。M-CASS具有较高的内部一致性（Cronbach alpha = 0.97），具有较高的一致性，类内相关系数为0.91。血浆细胞因子和氧化应激标志物水平均与M-CASS评分相关（Kruskal-Wallis检验，P < 0.05）。M-CASS允许跟踪疾病进展和动物福利要求。
With animal models, death as an intentional end point is ethically unacceptable. However, in the study of septic shock, death is still considered the only relevant end point. We defined eight humane end points into four stages of severity (from healthy to moribund) and used to design a clinically relevant scoring tool, termed "the mouse clinical assessment score for sepsis" (M-CASS). The M-CASS was used to enable a consistent approach to the assessment of disease severity. This allowed an ethical and objective assessment of disease after which euthanasia was performed, instead of worsening suffering. The M-CASS displayed a high internal consistency (Cronbach alpha = 0.97) with a high level of agreement and an intraclass correlation coefficient equal to 0.91. The plasma levels of cytokines and markers of oxidative stress were all associated with the M-CASS score (Kruskal-Wallis test, P < 0.05). The M-CASS allows tracking of disease progression and animal welfare requirements.