Effects of in vitro saliva, gastric and intestinal digestion on the chemical properties, antioxidant activity of polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp
Effects of in vitro saliva, gastric and intestinal digestion on the chemical properties, antioxidant activity of polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp
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DOI：
10.1016/j.foodhyd.2018.09.014
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发表时间：
2019-02
影响因子：
10.7
通讯作者：
Kexue Zhu;Siwen Yao;Zhang Yanjun;Qibing Liu;Fei Xu;Gang Wu;Wenjiang Dong;L. Tan
中科院分区：
文献类型：
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作者：
Kexue Zhu;Siwen Yao;Zhang Yanjun;Qibing Liu;Fei Xu;Gang Wu;Wenjiang Dong;L. Tan
多糖是植物、动物和微生物中分布最广、含量最丰富的化合物之一。在本研究中，我们研究了在体外唾液、胃和肠道消化中对异叶罗汉果多糖还原糖含量、相对分子质量和结构变化的影响。纸浆(JFP-Ps)。对消化后的JFP-Ps进行了DPPH清除实验、羟基自由基清除实验、超氧阴离子自由基清除实验和铁还原能力实验。结果表明，在体外消化条件下，JFP-Ps还原糖含量显著增加，相对分子质量显著降低。FT-IR结果表明，模拟消化后，JFP-Ps的结构和构象发生了明显的变化。JFP-Ps的肠道消化样品在消化过程中表现出明显的DPPH、羟基自由基和超氧阴离子自由基的清除活性，但还原能力相对较低。这些结果表明，消化显著地影响了JFP-Ps在胃肠道消化过程中的化学性质、结构和抗氧化活性，并可能有助于最大限度地将JFP-Ps作为一种在胃肠道消化过程中存活下来的抗氧化植物化学物质的膳食来源。
Polysaccharides are one of the most widely distributed and abundant compounds in plants, animals and microorganisms. In this study, we investigated the effects ofin vitrosaliva, gastric and intestinal digestion on the reducing sugar contents, molecular weight and structure change of polysaccharide fromArtocarpus heterophyllusLam. Pulp (JFP-Ps). Digested samples of JFP-Ps were also subject to antioxidant assays including DPPH scavenging assay, hydroxyl radical scavenging assay, superoxide radical scavenging assay and ferric reducing power assay. Results revealed thatin vitrodigestion could significantly increase the reducing sugar contents and decrease the molecular weight of JFP-Ps. FT-IR results showed that structure and conformation of JFP-Ps were remarkably altered after simulated digestion. The intestinal digested samples of JFP-Ps exhibited obvious DPPH, hydroxyl radical, and superoxide radical scavenging activities in a time-dependent manner during digestion, but with a relatively low reducing power. These results indicated that digestion remarkably affected the chemical properties, structure and antioxidant activity of JFP-Ps during gastrointestinal digestion, and might help maximize the potential applications of JFP-Ps as a dietary source of antioxidant phytochemicals that survive the gastrointestinal digestion process.