Xenografts from a human colon carcinoma and its metastases: establishment, characterization and differences in the pattern of carbohydrate-binding proteins.
Xenografts from a human colon carcinoma and its metastases: establishment, characterization and differences in the pattern of carbohydrate-binding proteins.
复制标题
人类结肠癌及其转移的异种移植物：碳水化合物结合蛋白模式的建立、表征和差异。
作者：
K. Vehmeyer;E. Kunze;T. Ciesiolka;G. Nagel;H. Gabius
通过合适的人体细胞生物学模型系统，可以在实验上研究人类结直肠癌转移的发病机制。这些研究的目的是建立人结肠癌及其转移的裸鼠移植瘤作为合适的模型。手术切除的结肠腺癌(3级)活检标本和两年后局部复发的小肠转移被建立为异种移植，并检查了它们的生长特征。两种组织类型在标记物表达方面有共同的特征(癌胚抗原、神经元特异性烯醇化酶、细胞角蛋白)。原发肿瘤有明显的坏死性渗出，细胞毒活性在几代后消失。内源性碳水化合物结合蛋白(凝集素)是公认的蛋白质-碳水化合物相互作用中与转移形成潜在相关的细胞糖偶联物的受体，其图谱揭示了这两个来源相同的人类肿瘤样本之间的差异，特别是在β-半乳糖苷特异性受体方面。这种糖生化分析采用了标准化的程序。与其他物种相比，传代时间过长也会导致侧貌的改变。这些研究可能会鼓励在同一患者中应用原发肿瘤及其转移系统，试图将细胞特征的表达与人结肠肿瘤细胞的生物学和临床行为联系起来。
Investigation of the pathogenesis of human colorectal carcinoma metastasis can be rendered experimentally possible by suitable human cell biological model systems. The purpose of these studies was to establish xenografts in nude mice from human colon carcinoma and from its metastasis in the same patient as an appropriate model. Surgically removed biopsy specimens from a colon adenocarcinoma (grade 3) and its local relapse two years later with metastases in the small intestine were established as xenotransplants and their growth characteristics examined. Both tissue types shared common characteristics with respect to marker expression (carcinoembryonic antigen, neuron-specific enolase, cytokeratin). The primary tumor showed remarkable development of necrotic effusion with cytotoxic activity that ceased after several passages. The profile of endogenous carbohydrate-binding proteins (lectins), the receptors for cellular glycoconjugates in a recognitive protein-carbohydrate interplay with potential relevance to metastases formation, revealed differences between these two human tumor samples of identical origin, especially with respect to beta-galactoside-specific receptors. This glycobiochemical analysis employed standardized procedures. Prolonged passaging was also shown to result in profile alterations, as was similarly noted in comparison to another species. These studies may encourage the application of systems of primary tumor and its metastases in the same patient in attempts to correlate the expression of cellular characteristics with the biological and clinical behavior of human colonic tumor cells.