Expression of prostaglandin G/H synthase-1 and -2 protein in human colon cancer.
Expression of prostaglandin G/H synthase-1 and -2 protein in human colon cancer.
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发表时间：
1995-06
期刊：
影响因子：
11.2
通讯作者：
S. Kargman;G. O'neill;P. Vickers;Jilly F. Evans;J. Mancini;S. Jothy
中科院分区：
文献类型：
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作者：
S. Kargman;G. O'neill;P. Vickers;Jilly F. Evans;J. Mancini;S. Jothy
前列腺素G/H合成酶（Prostaglandin G/H synthase， PGHS）是导致前列腺素形成的关键酶，是非甾体类抗炎药物的靶标。已经鉴定出两种形式的酶，PGHS-1和PGHS-2。流行病学证据表明，阿司匹林和其他非甾体类抗炎药可能降低结直肠癌的风险。我们用免疫印迹法检测了人类PGHS-1和PGHS-2蛋白在25例匹配的结肠癌和非肿瘤组织、4例癌前息肉、5例非癌患者对照结肠组织和3例匹配的正常和癌性乳腺组织中的表达。正常组织和肿瘤组织均检测到PGHS-1。25例正常结肠组织中23例未检测到PGHS-2，而25例结肠肿瘤中19例检测到PGHS-2。PGHS-2蛋白在4个人类癌前息肉样本、来自非癌症患者的对照结肠或匹配的正常或癌性乳腺组织中未被观察到。这些结果提示，非甾体类抗炎药对结肠癌的有益作用可能是通过抑制PGHS-2介导的。
Prostaglandin G/H synthase (PGHS), a key enzyme leading to the formation of prostaglandins, is the target of nonsteroidal antiinflammatory drugs. Two forms of the enzyme have been identified, PGHS-1 and PGHS-2. Epidemiological evidence has suggested that aspirin and other nonsteroidal antiinflammatory drugs may reduce the risk of colorectal cancer. We examined by immunoblot analyses the expression of human PGHS-1 and PGHS-2 protein in 25 matched colon cancer and nontumor tissues, 4 premalignant polyps, 5 control colon tissues from noncancer patients, and 3 matched normal and cancerous breast tissue samples. PGHS-1 was detected in all normal and tumor tissue. In contrast, PGHS-2 was not detected in 23 of 25 normal colon tissues but was detected in 19 of 25 colon tumors. PGHS-2 protein was not observed in four human premalignant polyp samples, control colon from noncancer patients, or matched normal or cancerous breast tissues. These results suggest that the beneficial effects of nonsteroidal antiinflammatory drugs in colon cancer may be mediated by inhibition of PGHS-2.