Genome sequence of Vibrio splendidus: an abundant planctonic marine species with a large genotypic diversity
Genome sequence of Vibrio splendidus: an abundant planctonic marine species with a large genotypic diversity
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DOI：
10.1111/j.1462-2920.2009.01918.x
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发表时间：
2009-08-01
影响因子：
5.1
通讯作者：
Mazel, Didier
中科院分区：
文献类型：
--
作者：
Le Roux, Frederique;Zouine, Mohamed;Mazel, Didier
华丽弧菌是海水中的优势弧菌物种，具有显著的遗传多样性；有几种菌株与无脊椎动物的死亡有关。我们报道了牡蛎病原菌华丽弧菌LGP32的全基因组序列，并与相关菌株的部分基因组序列进行了比较。如同该属的典型，华丽弧菌LGP32含有两条染色体(3.29和1.67Mb)，大多数重要的细胞过程由1号染色体编码。与另外两个华丽弧菌的部分基因组序列(菌株12B01和Med222)的比较证实了该物种中存在着高度的基因多样性，并导致了许多菌株特异性区域的鉴定，这些区域往往不能被指定为特定的重组机制。令人惊讶的是，染色体整合子是迄今为止分析的所有其他弧菌物种中变异最大的遗传元件，在12B01中缺失，并被Med222中的一个可移动元件灭活，而在LGP32中，它只包含有限数量的盒。最后，我们发现LGP32整合子含有一个新的dfrA盒，与革兰氏阴性临床分离株耐药整合子中的dfrA盒相关。这些结果表明，海洋弧菌可能是抗生素耐药性基因的来源。
P>Vibrio splendidus is a dominant Vibrio species in seawater presenting a remarkable genetic diversity; several strains have been linked to invertebrate's mortality. We report the complete genome sequence of V. splendidus LGP32, an oyster pathogen, and its comparison with partial genome sequences from related strains. As is typical for the genus, V. splendidus LGP32 contains two chromosomes (3.29 and 1.67 Mb) and most essential cellular processes are encoded by chromosome 1. Comparison with two other V. splendidus partial genome sequences (strains 12B01 and Med222) confirms the previously suggested high genotypic diversity within this species and led to the identification of numerous strain-specific regions that could frequently not be assigned to a specific mechanisms of recombination. Surprisingly, the chromosomal integron, the most variable genetic element in all other Vibrio species analysed to date, is absent from 12B01 and inactivated by a mobile element in Med222, while in LGP32 it only contains a limited number of cassettes. Finally, we found that the LGP32 integron contains a new dfrA cassette, related to those found in resistance integrons of Gram-negative clinical isolates. Those results suggest that marine Vibrio can be a source of antibiotic resistance genes.