Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements.
Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements.
复制标题
将功能分配给基因：基于转录后控制元件的Leishmania Major中S期表达基因的鉴定。
DOI：
10.1093/nar/gki742
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发表时间：
2005
影响因子：
14.9
通讯作者：
Shlomai J
中科院分区：
文献类型：
--
作者：
Zick A;Onn I;Bezalel R;Margalit H;Shlomai J
为基因分配功能是后基因组时代的主要挑战之一。通常，功能的分配是基于编码序列与已知基因序列的相似性，或者通过识别已知与特定途径或条件相关的转录顺式调控元件来分配的。在锥虫中，基因表达的调节主要发生在转录后水平，需要新的功能分配方法。在这里，我们证明了在大利什曼原虫中基于转录后控制元件的新的S期表达基因的鉴定，该元件被认为参与了几个核和线粒体S期表达基因的细胞周期依赖性表达。假设在主要乳杆菌中也存在类似的调控机制，我们对主要乳杆菌基因组中类似的控制元件进行了计算搜索。我们的计算扫描产生了132个基因，其中33%是已知DNA代谢基因的同源基因，63%没有任何注释。对其中7个基因的实验测试表明，它们的mRNAs在整个细胞周期中循环，在S阶段或之前达到最大水平。提示筛选与特定功能相关的转录后调控元件为锥虫基因的功能分配提供了一种有效的方法。
Assigning functions to genes is one of the major challenges of the post-genomic era. Usually, functions are assigned based on similarity of the coding sequences to sequences of known genes, or by identification of transcriptional cis-regulatory elements that are known to be associated with specific pathways or conditions. In trypanosomatids, where regulation of gene expression takes place mainly at the post-transcriptional level, new approaches for function assignment are needed. Here we demonstrate the identification of novel S-phase expressed genes in Leishmania major, based on a post-transcriptional control element that was recognized in Crithidia fasciculata as involved in the cell cycle-dependent expression of several nuclear and mitochondrial S-phase expressed genes. Hypothesizing that a similar regulatory mechanism is manifested in L.major, we have applied a computational search for similar control elements in the genome of L.major. Our computational scan yielded 132 genes, of which 33% are homologues of known DNA metabolism genes and 63% lack any annotation. Experimental testing of seven of these genes revealed that their mRNAs cycle throughout the cell cycle, reaching a maximum level during S-phase or just prior to it. It is suggested that screening for post-transcriptional control elements associated with a specific function provides an efficient method for assigning functions to trypanosomatid genes.
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作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
Abu-Elneel, K;Robinson, D R;Drew, M E;Englund, P T;Shlomai, J
通讯作者：
Shlomai, J