Time course of collagen and decorin changes in rat cardiac and skeletal muscle post-MI.
Time course of collagen and decorin changes in rat cardiac and skeletal muscle post-MI.
复制标题
DOI：
10.1152/ajpheart.2001.281.4.h1816
复制
发表时间：
2001-10
期刊：
影响因子：
--
通讯作者：
S. Zimmerman;D. Thomas;S. Velleman;X. Li;T. R. Hansen;R. McCormick
中科院分区：
文献类型：
--
作者：
S. Zimmerman;D. Thomas;S. Velleman;X. Li;T. R. Hansen;R. McCormick
我们研究了心肌梗死后（MI后）左心室（LV）中主要纤维性前胶原的信息（I型和III型mRNA）与随后胶原增生、交联和核心蛋白聚糖表达之间的时间关系。我们试图确定1）蛋白聚糖核心蛋白聚糖在已知作为MI的结果发生的细胞外基质（ECM）重塑中起什么作用和2）MI后早期骨骼肌ECM改变的程度。因此，在手术诱导产生小至中等大小的梗死（约LV质量的20%）后，在剩余的存活LV游离壁和慢[比目鱼肌（SOL）]和快收缩[腓肠肌（GAST）]骨骼肌中评价ECM重塑的程度和时间过程。在非梗死对照组和MI后72 h、1、2、5和13 wk的LV中测定核心蛋白聚糖、胶原和羟赖氨酰吡啶交联浓度以及α 1（I）（I型）和α 1（III）（III型）前胶原mRNA。除13周外，在所有时间点收集SOL和GAST肌肉的这些相同数据。I型前胶原mRNA在72小时和1周的时间点增加LV。III型前胶原mRNA在MI后1周升高，在MI后2周恢复至基线水平。胶原蛋白浓度显着增加1周，5周增加一倍以上，并在13周升高129%，在剩余的活LV。LV核心蛋白聚糖表达在早期时间点不变，但在MI后5周增加了38%，在MI后13周增加了一倍。在骨骼肌中，SOL和GAST肌肉中的前胶原mRNA发生短暂改变，对测量的ECM参数无任何明显影响。本研究首次报道了核心蛋白聚糖的上调时间过程及其与MI后存活心肌中HP交联和胶原积累增加的关系。
We examined the temporal relationship between messages (type I and type III mRNAs) for the principal fibrillar procollagens and subsequent collagen accretion, cross-linking, and decorin expression in the left ventricle (LV) postmyocardial infarction (post-MI). We sought to determine 1) what role the proteoglycan decorin plays in extracellular matrix (ECM) remodeling known to take place as a consequence of MI and 2) the extent skeletal muscle ECM is altered early post-MI. Therefore, after surgically induced production of small- to moderate-sized infarcts (approximately 20% of LV mass), extent and time course of ECM remodeling was evaluated in remaining viable LV free wall and in slow- [soleus (SOL)] and fast-twitch [gastrocnemius (GAST)] skeletal muscles. Decorin, collagen, and hydroxylysylpyridinium cross-link concentrations and alpha1(I) (type I) and alpha1(III) (type III) procollagen mRNAs were measured in LVs from noninfarcted controls and at 72 h, 1, 2, 5, and 13 wk post-MI. These same data were collected in SOL and GAST muscles at all time points except 13 wk. Type I procollagen mRNA increased at both 72-h and 1-wk time points in LVs. Type III procollagen mRNA was elevated at 1 wk, returning to baseline by 2 wk post-MI. Collagen concentration was significantly increased by 1 wk, more than doubled by 5 wk, and was elevated 129% by 13 wk in the remaining viable LV. LV decorin expression was unaltered at early time points, but increased 38% at 5 wk post-MI and doubled by 13 wk post-MI. In skeletal muscle, procollagen mRNAs were transiently altered in SOL and GAST muscles without any demonstrable effect on the measured ECM parameters. This study reports, for the first time, the upregulation time course of decorin and its relationship to increased HP cross-linking and accumulation of collagen in viable myocardium post-MI.