High copper and iron levels and expression of Mn-superoxide dismutase in mutant rats displaying hereditary hepatitis and hepatoma (LEC rats).
High copper and iron levels and expression of Mn-superoxide dismutase in mutant rats displaying hereditary hepatitis and hepatoma (LEC rats).
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表现出遗传性肝炎和肝癌的突变大鼠（LEC 大鼠）中铜和铁的高水平以及锰超氧化物歧化酶的表达。
DOI：
10.1093/carcin/14.9.1881
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发表时间：
1993
期刊：
影响因子：
4.7
通讯作者：
Naoyuki Taniguchi
中科院分区：
文献类型：
--
作者：
Keiichiro Suzuki;Nobuko Miyazawa;T. Nakata;Han Geuk Seo;Toshihiro Sugiyama;Naoyuki Taniguchi
LEC大鼠是一种表现遗传性肝炎和肝癌的突变品系。我们建立了Cu，Zn-和Mn-超氧化物歧化酶（Cu，Zn-和Mn-SOD）的酶联免疫吸附测定法，并测定了LEC和Wistar大鼠各器官中SOD的水平。LEC大鼠各组织中Mn-SOD含量均高于Wistar大鼠。LEC大鼠肝、肾、肠组织Cu，Zn-SOD含量均低于Wistar大鼠。原子吸收技术表明，除了高铜浓度如前所述，LEC大鼠肝脏中含有较高的铁浓度相对于Wistar大鼠肝脏。这些数据表明，增加铁和铜的浓度和降低水平的Cu，Zn-SOD可能会促进芬顿反应，产生羟自由基的LEC大鼠的组织。在LEC大鼠肝炎和肝癌发生过程中，Mn-SOD可能发生适应性升高，以弥补Cu，Zn-SOD水平降低所致的清除作用的降低。
The LEC rat is a mutant strain displaying hereditary hepatitis and hepatoma. We established enzyme-linked immunosorbent assays of Cu,Zn- and Mn-superoxide dismutase (Cu,Zn- and Mn-SOD) and measured the levels of both SODs in various organs of LEC and Wistar rats. Mn-SOD concentrations were higher in LEC rats than in Wistar rats in most tissues. Cu,Zn-SOD levels of liver, kidney and intestine were lower in LEC rats than in Wistar rats. Atomic absorbtion techniques indicated that in addition to high Cu concentrations as previously reported, LEC rat livers contained high Fe concentrations relative to those in Wistar rat livers. These data suggest that increased concentrations of Fe and Cu and decreased levels of Cu,Zn-SOD may facilitate the Fenton reaction to produce hydroxyl radicals in the tissues of the LEC rat. To compensate for the decreased scavenging effects due to low levels of Cu,Zn-SOD, an adaptive increase of Mn-SOD may occur in the process of hepatitis and hepatocarcinogenesis in LEC rats.