Nervous system disruption and concomitant behavioral abnormality in early hatched pufferfish larvae exposed to heavy oil
Nervous system disruption and concomitant behavioral abnormality in early hatched pufferfish larvae exposed to heavy oil
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DOI：
10.1007/s11356-012-0833-0
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发表时间：
2012-08-01
影响因子：
5.8
通讯作者：
Murakami, Yasunori
中科院分区：
文献类型：
--
作者：
Kawaguchi, Masahumi;Sugahara, Yuki;Murakami, Yasunori
海洋上的重油（HO）泄漏已被证明对海洋生物有不利影响。据推测，暴露于HO的早期幼虫的沉卵在很大程度上导致正常的形态，而发育中的神经支架的异常组织是可能被发现的HO诱导的神经系统，控制动物的行为的中断，可能反过来导致异常的孵化幼虫的游泳行为。为阐明HO的毒理学效应，采用红鳍东方鲀（Takifugu rubripes）受精卵经50 mg/L HO处理8 d后，在孵化前将受精卵转移至新鲜海水中，进行了暴露试验和形态学及行为学分析。对孵化出的仔鱼的游泳行为、形态学特征、肌肉和神经系统的结构进行了观察，未发现HO暴露仔鱼的身体形态有任何异常。然而，他们表现出异常的游泳模式和混乱的中脑，一个更高的中心控制运动。我们的研究结果表明，HO暴露的鱼类遭受大脑发育障碍，引发异常的游泳行为，HO可能是选择性的大脑毒性，并导致身体残疾，在这些鱼类的整个生命周期。
Spills of heavy oil (HO) over the oceans have been proven to have an adverse effect on marine life. It has been hypothesized that exposure of early larvae of sinking eggs to HO leads largely to normal morphology, whereas abnormal organization of the developing neural scaffold is likely to be found. HO-induced disruption of the nervous system, which controls animal behavior, may in turn cause abnormalities in the swimming behavior of hatched larvae. To clarify the toxicological effects of HO, we performed exposure experiments and morphological and behavioral analyses in pufferfish (Takifugu rubripes) larvae.Fertilized eggs of pufferfish were exposed to 50 mg/L of HO for 8 days and transferred to fresh seawater before hatching. The hatched larvae were observed for their swimming behavior, morphological appearance, and construction of muscles and nervous system.In HO-exposed larvae, we did not detect any anomaly of body morphology. However, they showed an abnormal swimming pattern and disorganized midbrain, a higher center controlling movement. Our results suggest that HO-exposed fishes suffer developmental disorder of the brain that triggers an abnormal swimming behavior and that HO may be selectively toxic to the brain and cause physical disability throughout the life span of these fishes.