Mussel-inspired protein-mediated surface functionalization of electrospun nanofibers for pH-responsive drug delivery.
Mussel-inspired protein-mediated surface functionalization of electrospun nanofibers for pH-responsive drug delivery.
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DOI：
10.1016/j.actbio.2013.11.012
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发表时间：
2014-03
影响因子：
9.7
通讯作者：
Wang CH
中科院分区：
文献类型：
--
作者：
Jiang J;Xie J;Ma B;Bartlett DE;Xu A;Wang CH
pH响应性药物传递系统可以根据疾病的病理生理需要，在特定时间通过改变pH值来调节药物释放速率。在此，我们证明了贻贝启发的蛋白质聚多巴胺涂层可以调节静电纺聚（ε-己内酯）（PCL）纳米纤维在不同pH值溶液中的负载和释放率。体外释放谱显示，在相同的培养时间内，正电荷分子在酸性环境中的释放速度明显快于中性和碱性环境。双乙酸荧光素染色和3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）测定结果定性和定量地显示了不同pH浓度的阿霉素释放介质对癌细胞的杀伤作用，表明在低pH浓度下释放的含有阿霉素的培养液比在高pH浓度下释放的培养液杀死的细胞数量显著增加。总之，基于聚多巴胺包被PCL纳米纤维的ph响应药物递送系统在治疗胃癌的抗癌药物口服递送和抗病毒药物或抗炎药物的阴道递送中具有潜在的应用前景，可以提高药物的疗效，将药物递送到特定的靶点，并最大限度地减少毒副作用。
pH-responsive drug delivery systems could mediate drug releasing rate by changing pH values at specific time as per the pathophysiological need of the disease. Herein, we demonstrated a mussel inspired protein polydopamine coating can tune the loading and releasing rate of charged molecules from electrospun poly (ε-caprolactone) (PCL) nanofibers in solutions with different pH values. In vitro release profiles showed that the positive charged molecules released significantly faster in acidic than those in neutral and basic environments within the same incubation time. The results of fluorescein diacetate staining and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays showed the viability of cancer cells after treatment with doxorubicin released media at different pH values qualitatively and quantitatively, indicating the media contained doxorubicin which was released in solutions at low pH values could kill significantly higher number of cells than that released in solutions at high pH values. Together, the pH-responsive drug delivery systems based on polydopamine-coated PCL nanofibers could have potential applications in oral delivery of anticancer drugs for treating gastric cancer and vaginal delivery of anti-viral drugs or anti-inflammatory drugs, which could raise their efficacy, deliver them to the specific target, and minimize their toxic side effects.