Molecular profiling and the identification of genes associated with metastatic oral cavity/pharynx squamous cell carcinoma
Molecular profiling and the identification of genes associated with metastatic oral cavity/pharynx squamous cell carcinoma
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DOI：
10.1001/archotol.130.3.295
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发表时间：
2004-03-01
影响因子：
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通讯作者：
Hanash, S
中科院分区：
文献类型：
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作者：
Schmalbach, CE;Chepeha, DB;Hanash, S
目的：为了研究口腔/口咽鳞状细胞癌（OC/OP SCC）原发性肿瘤转移到颈部淋巴结和非转移性OC/OP SCC tumors.Design之间的基因表达谱的差异：原发性肿瘤的寡核苷酸微阵列分析被用来产生基因表达谱。转移性和非转移性肿瘤之间的配置文件进行比较，使用主成分分析，t检验，和倍数变化的差异。转移性肿瘤和非癌口腔粘膜样本之间进行了类似的比较，以确保肿瘤origin.Subjects：一个前瞻性队列的20例患者与以前未经治疗的OC/OP SCC谁经历了手术切除和淋巴结切除术后的病理分期。在大约9600个基因中，101在转移性和非转移性肿瘤之间表现出显著的表达差异（倍数变化大于或等于1.5; p= 1.5; P
Objective: To investigate differences in gene expression profiles between oral cavity/oropharynx squamous cell carcinoma (OC/OP SCC) primary tumors that have metastasized to cervical lymph nodes and nonmetastatic OC/OP SCC tumors.Design: Oligonucleotide microarray analysis of primary tumors was used to produce gene expression profiles. Profile comparisons between metastatic and nonmetastatic tumors were performed using principal component analysis, t test, and fold change differences. A similar comparison between metastatic tumors and noncancer oral mucosa samples was performed to ensure tumor origin.Subjects: A prospective cohort of 20 patients with previously untreated OC/OP SCC who underwent pathologic staging following surgical resection and lymphadenectomy.Results: Of the approximately 9600 genes profiled,101 demonstrated significant expression differences between the metastatic and nonmetastatic tumors (fold change greater than or equal to 1,5; p= 1.5; P