PV-1 is recognized by the PAL-E antibody and forms complexes with NRP-1
PV-1 is recognized by the PAL-E antibody and forms complexes with NRP-1
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DOI：
10.1182/blood-2012-01-406876
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发表时间：
2012-07-05
期刊：
影响因子：
20.3
通讯作者：
Jalkanen, Sirpa
中科院分区：
文献类型：
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作者：
Keuschnigg, Johannes;Tvorogov, Denis;Jalkanen, Sirpa
病理解剖学Leiden内皮（PAL-E）抗体作为血管内皮的原型标记物已经使用了20多年。该抗体的难以捉摸的靶点被认为是质膜囊泡相关蛋白-1（PV-1）和神经纤毛蛋白-1（NRP-1）。使用免疫荧光，我们表明，PAL-E，抗PV-1，抗NRP-1，抗CD 31抗体显示在不同组织的血管系统中的染色模式基本相同。然而，PV-1转染的细胞仅结合PAL-E和抗PV-1抗体，而NRP-1转染子在流式细胞术中用抗NRP-1抗体染色。使用来自组织和转染细胞的裂解物，我们进一步证实PAL-E和抗PV-1抗体识别的分子不是NRP-1而是PV-1。然而，免疫共沉淀研究明确表明，NRP-1可以与PV-1形成复合物。这首次将参与白细胞运输和血管生成的2种分子联系起来，从而为该领域的未来研究开辟了有趣的可能性。（血。2012;120（1）：232-235）
Pathologische anatomie leiden endothelium (PAL-E) antibody has been used for more than 20 years as a prototype marker for vascular endothelium. The elusive target of this antibody has been claimed to be plasmalemma vesicle-associated protein-1 (PV-1) and neuropilin-1 (NRP-1). Using immunofluorescence, we show that PAL-E, anti-PV-1, anti-NRP-1, and anti-CD31 antibodies show largely identical staining patterns in the vasculature of different tissues. However, PV-1-transfected cells only bind PAL-E and anti-PV-1 antibodies, whereas NRP-1 transfectants stain with anti-NRP-1 antibodies in flow cytometry. Using lysates from tissues and transfected cells, we further confirm that the molecule recognized by PAL-E and anti-PV-1 antibodies is not NRP-1 but PV-1. Nevertheless, coimmunoprecipitation studies unambiguously demonstrate that NRP-1 can form complexes with PV-1. This connects, for the first time, 2 molecules involved in leukocyte trafficking and angiogenesis, thereby opening interesting possibilities for future research in this field. (Blood. 2012;120(1):232-235)