INCIDENCE OF VIBRIO-PARAHAEMOLYTICUS IN UNITED-STATES COASTAL WATERS AND OYSTERS
INCIDENCE OF VIBRIO-PARAHAEMOLYTICUS IN UNITED-STATES COASTAL WATERS AND OYSTERS
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DOI：
10.1128/aem.56.8.2299-2302.1990
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发表时间：
1990-08-01
影响因子：
4.4
通讯作者：
MCPHEARSON, RM
中科院分区：
文献类型：
--
作者：
DEPAOLA, A;HOPKINS, LH;MCPHEARSON, RM
从1984年5月至1985年4月，从华盛顿、加州、得克萨斯、路易斯安那、亚拉巴马、佛罗里达、南卡罗来纳州、弗吉尼亚和罗得岛的贝类生长区（已被国家贝类卫生计划指定为批准或禁止），通过海洋采集牡蛎和海水样本。粪便大肠菌群计数，好氧平板计数，副溶血性弧菌密度进行了测定的样品。牡蛎中的平均副溶血性弧菌密度是水中的100倍以上，而粪便大肠菌群的密度大约是牡蛎中的10倍。副溶血性弧菌的季节和地理分布与水温有关，在春季和夏季沿着墨西哥湾沿岸采集的样本中密度最高。合成的耐热直接溶血素DNA探针与50株分离株中的2株杂交，其中1株经神奈川试验阳性。
Osyter and seawater samples were collected seaonally from May 1984 through April 1985 from shellfish-growing areas in Washington, California, Texas, Louisiana, Alabama, Florida, South Carolina, Virginia, and Rhode Island which has been designated as approved or prohibited by the National Shellfish Sanitation Program. Fecal coliforms counts, aerobic plate counts, and Vibrio parahaemolyticus densities were determined for the samples. Mean V. parahemaolyticus density was more than 100 times greater in oysters than in water, whereas density of fecal coliforms was approximately 10 times higher in osyters. Seasonal and geographical distribution of V. parahaemolyticus were related to water temperature, with highest densities in samples collected in the spring and the summer along the Gulf coast. The synthetic DNA probe for thermostable direct hemolysin hybridized with 2 of 50 isolates, 1 of which was positive by the Kanagawa test.