Antigenic cartography using variant-specific hamster sera reveals substantial antigenic variation among Omicron subvariants
Antigenic cartography using variant-specific hamster sera reveals substantial antigenic variation among Omicron subvariants
复制标题
使用变体特异性仓鼠血清进行的抗原制图揭示了 Omicron 亚变体之间的显着抗原变异
DOI：
10.1101/2023.07.02.547076
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
C. Drosten
中科院分区：
文献类型：
--
作者：
Barbara Mühlemann;J. Trimpert;Felix Walper;Marie L Schmidt;S. Schroeder;L. M. Jeworowski;J. Beheim;T. Bleicker;Daniela Niemeyer;J. Adler;Ricardo Martin Vidal;Christine Langner;Daria Vladimirova;Derek J. Smith;Mathias Voß;Lea Paltzow;Christina Martínez Christophersen;R. Rose;A. Krumbholz;T. Jones;V. Corman;C. Drosten
SARS-CoV-2已经发展出相当大的抗原变异性。由于大多数人群由于感染或疫苗接种而具有预先存在的免疫力，因此使用实验产生的动物免疫血清对于测量病毒变体之间的抗原差异可能是有价值的。在这里，我们用八种SARS-CoV-2变体中的一种连续两次感染来免疫叙利亚仓鼠。他们的血清针对14种SARS-CoV-2变异体进行滴定，并使用抗原制图法显示所得滴度。抗原图显示了一个包含所有前Omicron变体的浓缩簇（D 614 G，Alpha，Delta，Beta，Mu和工程B.1+ E484 K变体），以及在选定的Omicron子变体组中分布更广的定位（BA. 1、BA. 2、BA. 4/5、BA. 5后代BF. 7和BQ. 1.18; BA. 2.75后代BN.一些Omicron亚变体在抗原性上彼此不同，就像野生型与Omicron BA.1变体一样。结果突出了使用变异特异性感染的仓鼠血清用于SARS-CoV-2的持续抗原表征的潜力。
SARS-CoV-2 has developed substantial antigenic variability. As the majority of the population now has pre-existing immunity due to infection or vaccination, the use of experimentally generated animal immune sera can be valuable for measuring antigenic differences between virus variants. Here, we immunized Syrian hamsters by two successive infections with one of eight SARS-CoV-2 variants. Their sera were titrated against 14 SARS-CoV-2 variants and the resulting titers visualized using antigenic cartography. The antigenic map shows a condensed cluster containing all pre-Omicron variants (D614G, Alpha, Delta, Beta, Mu, and an engineered B.1+E484K variant), and a considerably more distributed positioning among a selected panel of Omicron subvariants (BA.1, BA.2, BA.4/5, the BA.5 descendants BF.7 and BQ.1.18; the BA.2.75 descendant BN.1.3.1; and the BA.2-derived recombinant XBB.2). Some Omicron subvariants were as antigenically distinct from each other as the wildtype is from the Omicron BA.1 variant. The results highlight the potential of using variant-specifically infected hamster sera for the continued antigenic characterisation of SARS-CoV-2.
影响因子：
64.5
作者：
Dejnirattisai W;Zhou D;Supasa P;Liu C;Mentzer AJ;Ginn HM;Zhao Y;Duyvesteyn HME;Tuekprakhon A;Nutalai R;Wang B;López-Camacho C;Slon-Campos J;Walter TS;Skelly D;Costa Clemens SA;Naveca FG;Nascimento V;Nascimento F;Fernandes da Costa C;Resende PC;Pauvolid-Correa A;Siqueira MM;Dold C;Levin R;Dong T;Pollard AJ;Knight JC;Crook D;Lambe T;Clutterbuck E;Bibi S;Flaxman A;Bittaye M;Belij-Rammerstorfer S;Gilbert SC;Carroll MW;Klenerman P;Barnes E;Dunachie SJ;Paterson NG;Williams MA;Hall DR;Hulswit RJG;Bowden TA;Fry EE;Mongkolsapaya J;Ren J;Stuart DI;Screaton GR
通讯作者：
Screaton GR