Transcriptome Profiling of the Rice Blast Fungus Magnaporthe oryzae and Its Host Oryza sativa During Infection
Transcriptome Profiling of the Rice Blast Fungus Magnaporthe oryzae and Its Host Oryza sativa During Infection
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DOI：
10.1094/mpmi-07-19-0207-a
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发表时间：
2020-02-01
影响因子：
3.5
通讯作者：
Lee, Yong-Hwan
中科院分区：
文献类型：
--
作者：
Jeon, Jongbum;Lee, Gir-Won;Lee, Yong-Hwan
稻瘟病(真菌病原菌：稻瘟菌和寄主：水稻)是研究植物-微生物相互作用的最重要的模式病理系统之一。虽然这两个基因组序列都是作为病原体和宿主的第一个病例发表的，但只有少数植物在感染期间的转录组数据中可用。由于技术上的困难，以前报道的真菌转录组数据不是很有资格全面描述真菌基因在感染过程中的表达。在这里，我们使用基于鞘感染的RNA测序方法报告了稻米和水稻在感染过程中的高质量转录本。这种对真菌病原菌及其寄主的全面表达谱将为在基因组水平上了解植物与微生物的相互作用提供更好的平台，并为研究界提供宝贵的资源。
The rice blast (fungal pathogen: Magnaporthe oryzae and host: Oryza sativa) is one of the most important model pathosystems for understanding plant-microbe interactions. Although both genome sequences were published as the first cases of pathogen and host, only a few in planta transcriptome data during infection are available. Due to technical difficulties, previously reported fungal transcriptome data are not highly qualified to comprehensively profile the expression of fungal genes during infection. Here, we report the high-quality transcriptomes of M. oryzae and rice during infection using a sheath infection-based RNA sequencing approach. This comprehensive expression profiling of the fungal pathogen and its host will provide a better platform for understanding the plant-microbe interactions at the genomic level and serve as a valuable resource for the research community.