Antagonism against Vibrio cholerae by diffusible substances produced by bacterial components of the human faecal microbiota.
Antagonism against Vibrio cholerae by diffusible substances produced by bacterial components of the human faecal microbiota.
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人类粪便微生物群的细菌成分产生的可扩散物质对抗霍乱弧菌。
DOI：
10.1099/0022-1317-50-2-161
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发表时间：
2001
影响因子：
3
通讯作者：
J. R. Nicoli
中科院分区：
文献类型：
--
作者：
Simone Helena Da Silva;E. Vieira;R. Dias;J. R. Nicoli
霍乱弧菌有时会存活在慢性携带者的小肠中，可能在低水平的沉默人群中生存，或者通过少数人的胃肠道传播，而不会引起腹泻或定居。为了了解这些情况，本研究使用平板培养(体外试验)来调查92名4-61岁的健康志愿者(女性40名，男性52名)的粪便标本产生的抑制霍乱弧菌晕圈的出现频率。全组抑制性晕圈发生率为20.6%。20~40岁组明显高于4~19岁组(10.7%)，41~61岁组无明显差异(20.0%)。男性(30.8%)明显高于女性(7.5%)。通过厌氧室中十进制稀释液的平板培养，一名志愿者的粪便产生抑制光环的优势微生物区系被分离出来。26个形态明显不同的潜在分离株与无菌NIH小鼠相关。一周后，抑制试验显示，相关动物的粪便周围出现了拮抗光环，但无菌小鼠的粪便没有。在分离到的26株细菌中，有2株(乳杆菌属Lactobacillussp.和消化链球菌属(PectoStrechain Sp.)在体外试验中产生了一种对霍乱弧菌的复合拮抗剂。当与无菌小鼠进行双向关联时，这些菌株在大约5天内消除了肠道生态系统中的弧菌。
Cholera vibrios sometimes survive, probably in low-level silent populations, in the small intestine of chronic carriers or pass through the gastrointestinal tract of a few individuals without causing diarrhoea or colonisation. To understand these situations, the present study used plate cultures (ex-vivo test) to investigate the frequency of appearance of an inhibitory halo against Vibrio cholerae produced by faecal specimens from 92 healthy volunteers (40 females, 52 males) aged 4-61 years. The frequency of inhibitory halo was 20.6% in the whole group. An apparently higher percentage (27.3%) was observed in the age range 20-40 years when compared with the range 4-19 years (10.7%), but not the range 41-61 years (20.0%). Frequency was significantly higher in males (30.8%) than females (7.5%). The dominant microbiota of a volunteer whose faeces produced an inhibitory halo was isolated by plate culture of decimal dilutions in an anaerobic chamber. Potential isolates of 26 apparently different morphologies were associated with germ-free NIH mice. One week later, the inhibitory test showed an antagonistic halo around the faeces from the associated animals, but not from the axenic mice. Of the 26 bacteria isolated, two (Lactobacillus sp. and Peptostreptococcus sp.) produced a compound antagonistic against V. cholerae in an in-vitro assay. When bi-associated with germ-free mice those strains eliminated the vibrio from the intestinal ecosystem in c. 5 days.