ISOLATION AND CHARACTERIZATION OF MICROSATELLITE LOCI IN RHODODENDRON FERRUGINEUM (ERICACEAE) USING PYROSEQUENCING TECHNOLOGY
ISOLATION AND CHARACTERIZATION OF MICROSATELLITE LOCI IN RHODODENDRON FERRUGINEUM (ERICACEAE) USING PYROSEQUENCING TECHNOLOGY
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DOI：
10.3732/ajb.1000533
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发表时间：
2011-05-01
影响因子：
3
通讯作者：
Escaravage, Nathalie
中科院分区：
文献类型：
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作者：
Delmas, Chloe E. L.;Lhuillier, Emeline;Escaravage, Nathalie
。研究前提：为杜鹃花（杜鹃花科）开发微卫星引物，以评估该自交亲和物种的遗传多样性、种群遗传结构和交配系统。方法与结果：采用新一代454 FLX钛焦磷酸测序技术分离出102个新的微卫星位点。两个多重 PCR 组经过优化，可对 9 个多态性微卫星位点进行基因分型。对来自比利牛斯山脉（法国）的两个种群的遗传多样性水平进行了评估。每个基因座的平均等位基因数范围为 1.5 至 7。观察到的平均杂合度和预期杂合度的范围分别为 0 至 0.76 和 0.03 至 0.66。对其他 13 个杜鹃花属和杜鹃花科的另外两个属进行了成功的跨物种扩增，平均每个物种扩增了 7 对引物。 结论：这些标记将有助于进一步研究杜鹃花科大科，特别是杜鹃花的进化史。
. Premise of the study: Microsatellite primers were developed for Rhododendron ferrugineum (Ericaceae) to evaluate genetic diversity, population genetic structure, and mating system of this self-compatible species.. Methods and Results: The new-generation 454 FLX Titanium pyrosequencing technology was used to isolate 102 novel microsatellite loci. Two multiplex PCR sets were optimized to genotype nine polymorphic microsatellite loci. The level of genetic diversity was assessed in two populations from the Pyrenees (France). The mean number of alleles per locus ranged from 1.5 to 7. The mean observed and expected heterozygosities ranged from 0 to 0.76 and from 0.03 to 0.66, respectively. Cross-species amplification was successful for 13 other Rhododendron species and two additional genera of Ericaceae, with an average of seven pairs of primers amplifying per species.. Conclusions : These markers will facilitate further studies on the evolutionary history of the large Ericaceae family and Rhododendron in particular.