Decreased density of interstitial cells of Cajal and neuronal cells in patients with slow-transit constipation and acquired megacolon
Decreased density of interstitial cells of Cajal and neuronal cells in patients with slow-transit constipation and acquired megacolon
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DOI：
10.1111/j.1440-1746.2005.03809.x
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发表时间：
2005-08-01
影响因子：
4.1
通讯作者：
Talbot, IC
中科院分区：
文献类型：
--
作者：
Lee, JI;Park, H;Talbot, IC
背景资料：便秘的病理生理机制尚不清楚，已知产生慢波活动并参与肠神经传递和肠神经系统（ENS）的Cajal细胞（ICC）被怀疑起重要作用。本研究的目的是评估ICC和神经元细胞的ENS在慢传输型便秘和后天性巨结肠患者的分布方法：乙状结肠标本来自结肠切除术，由于慢传输型便秘（n = 10），后天性巨结肠（n = 9）和非梗阻性结肠癌（n = 10）作为对照组。ICC通过c-Kit免疫组化显示，ENS的神经元细胞通过蛋白基因产物（PGP）9.5显示。用c-Kit和PGP 9.5染色的细胞密度计算为面积百分比（染色面积/X-Y平面面积）× 100，当以40倍物镜的放大率收集图像时，使用图像分析仪在400 μ m × 400 μ m的水平X-Y平面中检查最大面积。与对照组相比，慢传输型便秘和获得性巨结肠患者乙状结肠标本各层ICC和PGP9.5反应性神经元结构的密度均显著降低。结论：慢传输型便秘和获得性巨结肠患者乙状结肠内胆管细胞和ENS神经元结构的改变与其ICC密度和ENS神经元结构的改变有关。(C)2005年Blackwell Publishing Asia Pty Ltd.
Background: The pathophysiology of constipation is not clearly identified as yet, and the interstital cells of Cajal (ICC), known to generate the slow wave activity and to be involved in intestinal neurotransmission and the enteric nervous system (ENS), are suspected to play an important role. The aims of the present study were to assess the distribution of ICC and neuronal cells of ENS in patients with slow-transit constipation and acquired megacolon.Methods: Sigmoid colon specimens were obtained from patients who underwent colectomy due to slow-transit constipation (n = 10), acquired megacolon (n = 9) and non-obstructive colon cancer (n = 10) as a control group. The ICC were visualized by c-Kit immunohistochemistry and neuronal cells of the ENS were demonstrated by protein gene product (PGP) 9.5. Density of cells stained by c-Kit and PGP 9.5 was calculated as percent area (area stained/area of X-Y plane) X 100, when images were collected at a magnification of x40 objective, with maximum area examined in the horizontal X-Y plane of 400 mu m x 400 mu m using an image analyzer.Results: The densities of ICC and PGP 9.5 reactive neuronal structures were significantly decreased in all layers of sigmoid colon specimens in patients with slow-transit constipation and acquired megacolon, compared with that of the control group. However, there was no statistically significant difference in either the density of ICC or that of neuronal structures between the patients with slow-transit constipation and acquired megacolon.Conclusions: Slow-transit constipation and acquired megacolon were associated with alteration of ICC and neuronal cells of ENS in the sigmoid colon. (C) 2005 Blackwell Publishing Asia Pty Ltd.