Effect of bone-derived growth factor on DNA, RNA, and proteoglycan synthesis in cultures of rabbit costal chondrocytes.
Effect of bone-derived growth factor on DNA, RNA, and proteoglycan synthesis in cultures of rabbit costal chondrocytes.
复制标题
骨源性生长因子对兔肋软骨细胞培养物中 DNA、RNA 和蛋白聚糖合成的影响。
DOI：
10.1016/0026-0495(82)90080-4
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Canalis,E
中科院分区：
文献类型：
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作者：
Kato,Y;Watanabe,R;Nomura,Y;Tsuji,M;Suzuki,F;Raisz,LG;Canalis,E
据报道，培养的颅骨和软骨细胞分别释放刺激骨和软骨生长的生长因子。在本研究中，我们研究了骨源性生长因子（BDGF）对培养兔软骨细胞DNA、RNA和蛋白多糖合成的影响。对BDGF的两种部分纯化组分进行了测试，其中一种分子量约为20-30,000，对头颅DNA标记（BDGF I）具有较大的活性，另一种分子量约为6-13,000，对骨胶原标记（BDGF II）具有较大的活性。两种组分都有相似的效果，并且增加了- 3h -尿苷在软骨细胞中酸不溶性残基中的掺入，以及35so42 -、3h -氨基葡萄糖和3h -丝氨酸在蛋白聚糖中的掺入。然而，BDGF II对3h -胸腺嘧啶掺入的刺激作用比BDGF i更大。这些发现表明骨细胞释放的因子能够刺激软骨代谢和生长。
Calvariae and chondrocytes in culture have been reported to release growth factors which stimulate bone and cartilage growth respectively. In the present studies, we examined the effects of bone-derived growth factor (BDGF) on DNA, RNA and proteoglycan synthesis in cultured rabbit chondrocytes. Two partially purified fractions of BDGF were tested, one with an approximate molecular weight (MW) of 20–30,000 and with greater activity on calvarial DNA labeling (BDGF I) and another with an approximate MW 6–13,000 and greater activity on bone collagen labeling (BDGF II). Both fractions had a similar effect and increased the incorporation of−3H-uridine into acid insoluble residues in chondrocytes and the incorporation of35SO42-,3H-glucosamine and3H-serine into proteoglycans. However, BDGF II had a greater stimulatory effect on the incorporation of3H-thymidine than BDGF I. These findings suggest that factor(s) released by bone cells are capable of stimulating cartilage metabolism and growth.