High-resolution RFLP mapping of the fertility restoration (Rf3) gene against Triticum timopheevi cytoplasm located on chromosome 1BS of common wheat
High-resolution RFLP mapping of the fertility restoration (Rf3) gene against Triticum timopheevi cytoplasm located on chromosome 1BS of common wheat
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DOI：
10.1266/ggs.72.353
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发表时间：
1997-12-01
影响因子：
1.1
通讯作者：
Ogihara, Y
中科院分区：
文献类型：
--
作者：
Kojima, T;Tsujimoto, H;Ogihara, Y
通过中国春与 (timopheevi)-CS (雄性不育) 和 T. spelta (携带 Rf3) 之间的 F-1 植物连续回交，开发了针对提莫菲维 (Triticum timopheevi) 细胞质控制育性恢复的 Rf3 基因的异质小麦近等基因系。将所得的三株BC3F1植物进行自花授粉以获得125株BC3F2后代。利用这个群体，我们将 Rf3 基因精确定位在 RFLP 连锁图谱的小麦 1B 号染色体 (1BS) 的短臂上。 Rf3基因定位于分别距离Xcdo388和Xabc156 1.2cM和2.6cM的位置。经计算，Rf3 与 Nor 和 Gli-BI 的遗传距离分别为 22.3 cM 和 18.6 cM，支持了之前的数据。对该区域物理距离的估计表明，Rf3 基因与相邻 RFLP 标记的距离在 500 kbp 以内。除 Xbcd98 的位置外，遗传图谱的标记顺序与细胞学图谱的标记顺序相对应。遗传图谱和细胞学图谱之间的比较清楚地表明，RFLP 标记在整个染色体臂中分布不均匀，并且重组在染色体臂中发生的情况也不均匀。
Near-isogenic lines of alloplasmic wheat for the Rf3 gene controlling fertility restoration against the cytoplasm of Triticum timopheevi were developed by successive backcrossing of Chinese Spring to the F-1 plants between (timopheevi)-CS (male sterile) and T. spelta (carrying Rf3). The resultant three BC3F1 plants were self-pollinated so as to obtain 125 BC3F2 progenies. Using this population, we precisely mapped the Rf3 gene on the short arm of wheat chromosome 1B (1BS) of the RFLP linkage map. The Rf3 gene was localized at a position 1.2 cM and 2.6 cM distant from Xcdo388 and Xabc156, respectively. The genetic distances of Rf3 from Nor and Gli-BI were calculated to be 22.3 cM and 18.6 cM, respectively, supporting previous data. Estimation of the physical distance of the region suggets that the Rf3 gene resides within 500 kbp from the adjacent RFLP markers. The marker order of the genetic map corresponded to that of the cytological map, except for the position of Xbcd98. Comparison between genetic and cytological maps clearly shows that RFLP markers are unevenly distributed throughout the chromosome arm, and recombinations took place unequally in the chromosome arm.