REGENERATION OF LONG SPINAL AXONS IN THE RAT
REGENERATION OF LONG SPINAL AXONS IN THE RAT
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DOI：
10.1007/bf01148324
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
AGUAYO, AJ
中科院分区：
文献类型：
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作者：
RICHARDSON, PM;ISSA, VMK;AGUAYO, AJ
为研究大鼠脊髓长轴突的再生，在高颈、低颈、中胸或腰椎水平切断大鼠脊髓右侧柱，并在切口处将自体坐骨神经段的一端移植到脊髓上。用辣根过氧化物酶逆行追踪移植物内轴突的来源，有时顺行向脑干注射[~3H]氨基酸放射自显影示踪。在手术后。来自脑干各主要外侧脊髓束的轴突以及从下段脊髓上升的轴突成功地长出了低位颈椎移植物。长的下行轴突在中胸或腰椎损伤后很少再生；从脊髓的腰段上升的轴突通常无法进入高位颈椎移植物。在4个节段水平上轴突再生的差异不是简单地归因于纤维束中轴突数量的减少。从克拉克S柱或红核中观察到轴突再生，只有在损伤引起许多神经元萎缩的情况下才能观察到。在这些实验条件下，在脊髓外侧柱中没有明显的纤维束不能再生轴突，轴突再生得特别好。在这些实验条件下，细胞体到损伤的距离似乎是轴突再生的一个重要决定因素。
To investigate regeneration of long spinal axons, the right lateral column of the rat spinal cord was cut at high cervical, low cervical, midthoracic or lumbar level, and one end of an autologous sciatic nerve segment was grafted to the spinal cord at the site of incision. The origin of axons in the grafts was traced retrogradely with horseradish peroxidase injection into the grafts and, in some cases, anterogradely with radioautography of [3H] amino acids injected into the brainstem 3-6 mo. after the operation. Axons from each of the major lateral spinal tracts arising in the brainstem as well as axons ascending from the lower spinal cord succeeded in growing into low cervical grafts. Long descending axons rarely regenerated after midthoracic or lumbar injury; axons ascending from lumbar segments of the spinal cord usually failed to enter high cervical grafts. Differences in axonal regrowth at the 4 segmental levels were not simply attributable to dwindling of axonal number in fiber tracts. Axonal regeneration from Clarke''s column or the red nucleus was observed only with lesions causing atrophy of many neurons. There was no obvious example of a fiber tract in the lateral spinal columns from which axons failed to regenerate nor from which axons regenerated exceptionally well. Under the conditions of these experiments, the distance from cell body to injury aappeared to be an important determinant of axonal regeneration.