Distribution of allele frequencies at TTN g.231054C > T, RPL27A g.3109537C > T and AKIRIN2 c.*188G > A between Japanese Black and four other cattle breeds with differing historical selection for marbling.
Distribution of allele frequencies at TTN g.231054C > T, RPL27A g.3109537C > T and AKIRIN2 c.*188G > A between Japanese Black and four other cattle breeds with differing historical selection for marbling.
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DOI：
10.1186/1756-0500-4-10
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发表时间：
2011-01-20
影响因子：
1.8
通讯作者：
Yamada T
中科院分区：
文献类型：
--
作者：
Watanabe N;Satoh Y;Fujita T;Ohta T;Kose H;Muramatsu Y;Yamamoto T;Yamada T
由肌肉内脂肪的数量和分布定义的大理石纹，即所谓的大理石纹，是日本肉牛的一项重要经济性状。我们先前的研究在日本黑肉牛大理石花纹基因的5‘侧翼区(TTN)、核糖体蛋白L27a(RPL27A)的5’侧翼区(RPL27A)和akirin 2基因的3‘非翻译区(AKIRIN2)分别检测到3个单核苷酸多态(SNPs)，分别为g.231054C>；T、g.3109537C>；T和c.*188G>；A，并表明这些SNPs与日本黑肉牛大理石花纹存在关联。本研究调查了日本黑牛、日本棕牛、日本短刺牛、荷斯坦牛和瑞士棕牛5个品种的3个SNPs的等位基因频率分布。采用聚合酶链式反应-限制性片段长度多态性方法对日本短角牛14头、荷斯坦黑牛119头和瑞士棕牛118头公牛的TTN g.231054C>；T、RPL27A g.3109537C>；T和AKIRIN2 c.*188G>；A SNPs进行了基因分型。与与大理石花纹相关的g.231054C>；T、g.3109537C>；T和c.*188G>；A等位基因的频率相比，在受到高大理石花纹选择的日本黑色品种中，那些没有被选择为高大理石花纹的品种，日本短刺、荷斯坦和瑞士棕色品种中的那些没有被选择为高大理石花纹的品种为零或更低。小选大理石花纹的日本棕色品种的g.3109537C>；T等位基因频率低于日本黑色品种，而g.231054C>；T和c.*188G>；A等位基因的频率在两个品种之间没有差异。基于这一发现，我们推测日本黑种对高大理石花纹的强烈选择压力增加了TTN g.231054C>；T、RPL27A g.3109537C>；T和AKIRIN2 c.*188G>；A SNPs的T、T和A等位基因的频率。本研究与前人的关联研究表明，这3个SNPs可能有助于有效的标记辅助选择，以增加大理石花纹水平。
Marbling defined by the amount and distribution of intramuscular fat, so-called Shimofuri, is an economically important trait of beef cattle in Japan. Our previous study detected 3 single nucleotide polymorphisms (SNPs), g.231054C > T, g.3109537C > T and c.*188G > A, respectively, in the 5' flanking region of the titin (TTN), the 5' flanking region of the ribosomal protein L27a (RPL27A) and the 3' untranslated region of the akirin 2 genes (AKIRIN2), which have been considered as positional functional candidates for the genes responsible for marbling, and showed association of these SNPs with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the 3 SNPs among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Shorthorn, Holstein and Brown Swiss breeds. We genotyped the TTN g.231054C > T, RPL27A g.3109537C > T and AKIRIN2 c.*188G > A SNPs by polymerase chain reaction-restriction fragment length polymorphism method, using 101 sires and 1,705 paternal half sib progeny steers from 8 sires for Japanese Black, 86 sires and 27 paternal half sib progeny steers from 3 sires for Japanese Brown, 79 sires and 264 paternal half sib progeny steers from 14 sires for Japanese Shorthorn, 119 unrelated cows for Holstein, and 118 unrelated cows for Brown Swiss breeds. As compared to the frequencies of the g.231054C > T T, g.3109537C > T T and c.*188G > A A alleles, associated with high marbling, in Japanese Black breed that has been subjected to a strong selection for high marbling, those in the breeds, Japanese Shorthorn, Holstein and Brown Swiss breeds, that have not been selected for high marbling were null or lower. The Japanese Brown breed selected slightly for high marbling showed lower frequency than Japanese Black breed in the g.3109537C > T T allele, whereas no differences were detected between the 2 breeds in the frequencies of the g.231054C > T T and c.*188G > A A alleles. Based on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequencies of the T, T and A alleles at the TTN g.231054C > T, RPL27A g.3109537C > T and AKIRIN2 c.*188G > A SNPs, respectively. This study, together with the previous association studies, suggested that the 3 SNPs may be useful for effective marker-assisted selection to increase the levels of marbling.