Antibodies to Trichomonas vaginalis surface glycolipid.
Antibodies to Trichomonas vaginalis surface glycolipid.
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DOI：
10.1136/sextrans-2012-051013
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发表时间：
2013-09
影响因子：
3.6
通讯作者：
Corbeil LB
中科院分区：
文献类型：
--
作者：
Bastida-Corcuera FD;Singh BN;Gray GC;Stamper PD;Davuluri M;Schlangen K;Corbeil RR;Corbeil LB
人类滴虫病是最常见的非病毒性性传播疾病，但免疫反应没有得到很好的研究。由于迷走毛滴虫脂磷酸聚糖（TvLPG）是一个重要的毒力因子，一个银行的8个单克隆抗体产生的抗原在临床分离株定义。通过同种型特异性ELISA测定培养阳性（n=33）或阴性（n=33）的女性TvLPG特异性抗体应答。该银行的单克隆抗体与保守的表面TvLPG表位在27株孕妇在他们的第一次产前检查。通过免疫荧光显示保守的TvLPG表位是表面暴露的。在同一时间从同一患者收集的血清进行特异性抗体测定。在相同的临床研究中，来自33名T阴道炎阳性妇女的血清和阴道分泌物的IgG抗TvLPG水平在统计学上高于年龄匹配和种族匹配的阴性对照（p<0.01）。阴道伊加抗TvLPG水平的妇女滴虫病几乎显着高于对照组（p=0.055）。正常妊娠的感染妇女阴道IgG抗TvLPG值显著高于妊娠不良结局的感染妇女。这些抗体反应表明，感染的妇女可以响应保守的TvLPG抗原。由于滴虫表面LPG抗体在牛滴虫病模型中具有保护作用，因此应研究这些抗体在人类疾病中的作用。
Human trichomoniasis is the most common non-viral sexually transmitted disease, yet immune responses are not well studied. Since the Trichomonas vaginalis lipophosphoglycan (TvLPG) is an important virulence factor, a bank of eight monoclonal antibodies was generated to define the antigen in clinical isolates. The TvLPG-specific antibody response of women who were culture positive (n=33) or negative (n=33) for T vaginalis infection was determined by isotype-specific ELISA. The bank of monoclonal antibodies reacted with conserved surface TvLPG epitopes in 27 isolates from pregnant women at their first prenatal visit. Conserved TvLPG epitopes were shown to be surface exposed by immunofluorescence. Sera collected from the same patients at the same time were assayed for specific antibodies. Serum and vaginal secretions from 33 T vaginalis-positive women had statistically higher IgG anti-TvLPG levels than age-matched and race-matched negative controls in the same clinical study (p<0.01). Vaginal IgA anti-TvLPG levels of the women with trichomoniasis were almost significantly higher than controls (p=0.055). Infected women with normal pregnancies had significantly higher vaginal IgG anti-TvLPG values than infected women with adverse outcomes of pregnancy. These antibody responses show that infected women can respond to the conserved TvLPG antigen. Since antibodies to trichomonad surface LPG protect in a bovine model of trichomoniasis, the role of these antibodies in the human disease should be investigated.