Fully waterproof two-axis galvanometer scanner for enhanced wide-field optics optical-resolution photoacoustic microscopy
Fully waterproof two-axis galvanometer scanner for enhanced wide-field optics optical-resolution photoacoustic microscopy
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DOI：
10.1364/ol.380032
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发表时间：
2020-02-15
期刊：
影响因子：
3.6
通讯作者：
Kim, Jeehyun
中科院分区：
文献类型：
--
作者：
Lee, Jaeyul;Han, Sangyeob;Kim, Jeehyun
光声显微镜（PAM）由于其超声波信号传输系统采用淹没式结构，相对难以实现大视场和快速扫描。研究人员已经广泛研究了防水扫描仪的成就，用于动态生物应用，具有高分辨率和高信噪比。这封信报告了一种新的，据我们所知，防水振镜扫描仪为基础的PAM系统，成功地达到9.0毫米× 14.5毫米的扫描区域，幅度扫描速率为40千赫，空间分辨率为4.9 μ m。完整颅骨微血管可视化中小鼠大脑的体内表征证明了其在生物医学成像中的能力，并且预计将成为各种临床前和临床研究的有效技术。(C)2020美国光学学会
A large field-of-view and fast scanning of photoacoustic microscopy (PAM) relatively have been difficult to obtain due to the water-drowned structure of the system for the transmission of ultrasonic signals. Researchers have widely studied the achievement of a waterproof scanner for dynamic biological applications with a high-resolution and high signal-to-noise ratio. This Letter reports a novel, to the best of our knowledge, waterproof galvanometer scanner-based PAM system with a successfully attainable 9.0 mm x 14.5 mm scan region, amplitude scan rate of 40 kHz, and spatial resolution of 4.9 mu m. The in vivo characterization of a mouse brain in intact-skull microvascular visualization demonstrated its capability in biomedical imaging and is anticipated to be an effective technique for various preclinical and clinical studies. (C) 2020 Optical Society of America