Asymmetric Division and Differential Gene Expression during a Bacterial Developmental Program Requires DivIVA
Asymmetric Division and Differential Gene Expression during a Bacterial Developmental Program Requires DivIVA
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DOI：
10.1371/journal.pgen.1004526
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发表时间：
2014-08-01
期刊：
影响因子：
4.5
通讯作者：
Ramamurthi, Kumaran S.
中科院分区：
文献类型：
--
作者：
Eswaramoorthy, Prahathees;Winter, Peter W.;Ramamurthi, Kumaran S.
枯草芽孢杆菌中的产孢子是一个发育过程，在这个过程中，前体细胞分化成两种不同的细胞类型，其中较小的一种最终成为休眠细胞，称为孢子。这个过程始于不对称的细胞分裂事件，随后是转录因子sigma(F)的激活，特别是在较小的细胞中。在这里，我们证明了结构蛋白DivIVA在孢子形成过程中定位于极隔膜，是Sigma(F)的不对称分裂和隔室特异性激活所必需的。众所周知，这两种情况都需要一种名为SpoIIE的蛋白质，这种蛋白质也定位于极隔膜。我们发现DivIVA与SpoIIE交配，DivIVA可能会将SpoIIE短暂锚定到组装的极隔膜上，然后SpoIIE被释放到前孔膜并在极隔膜重新捕获。最后，使用超分辨率显微镜，我们证明DivIVA和SpoIIE最终在极隔的一侧显示出偏向的定位，面对激活sigma(F)的较小隔室。
Sporulation in the bacterium Bacillus subtilis is a developmental program in which a progenitor cell differentiates into two different cell types, the smaller of which eventually becomes a dormant cell called a spore. The process begins with an asymmetric cell division event, followed by the activation of a transcription factor, sigma(F), specifically in the smaller cell. Here, we show that the structural protein DivIVA localizes to the polar septum during sporulation and is required for asymmetric division and the compartment-specific activation of sigma(F). Both events are known to require a protein called SpoIIE, which also localizes to the polar septum. We show that DivIVA copurifies with SpoIIE and that DivIVA may anchor SpoIIE briefly to the assembling polar septum before SpoIIE is subsequently released into the forespore membrane and recaptured at the polar septum. Finally, using super-resolution microscopy, we demonstrate that DivIVA and SpoIIE ultimately display a biased localization on the side of the polar septum that faces the smaller compartment in which sigma(F) is activated.