Transcriptomic insights into the allelopathic effects of the garlic allelochemical diallyl disulfide on tomato roots
Transcriptomic insights into the allelopathic effects of the garlic allelochemical diallyl disulfide on tomato roots
复制标题
大蒜化感化学二烯丙基二硫化物对番茄根的化感作用的转录组学见解
DOI：
10.1038/srep38902
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发表时间：
2016-12-12
影响因子：
4.6
通讯作者：
Meng, Huan-Wen
中科院分区：
文献类型：
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作者：
Cheng, Fang;Cheng, Zhi-Hui;Meng, Huan-Wen
大蒜是一种化感作物，可以缓解蔬菜作物连作障碍。二烯丙基二硫（DADS）是大蒜中最重要的化感物质之一，对番茄根系生长有促进作用。因此，研究了DADS处理的番茄根随时间的总体转录组谱，以揭示潜在的生长促进机制。我们检测到1828，1296和1190差异表达基因（DEG）在4，24和48小时的样品，分别。短期（4 h）DADS处理后，大多数参与同化硫酸盐还原和谷胱甘肽代谢的DEGs上调。此外，还观察到防御酶活性的增加和6个过氧化物酶基因的上调，表明DADS可以诱导番茄抗性。在植物-病原体相互作用中，与钙信号相关的DEG主要受到抑制，而编码病程相关蛋白的DEG主要受到上调。DADS对植物激素合成和信号转导都有显著影响，但这两条途径中基因的表达趋势是相互矛盾的。该研究提供了有关DADS影响番茄根系转录组变化的全面信息，并可能有助于指导对DADS响应基因的进一步研究，以提高目前对DADS消除连作障碍的机制的理解。
Garlic is an allelopathic crop that can alleviate the obstacles to continuous cropping of vegetable crops. Diallyl disulfide (DADS), one of the most important allelochemicals in garlic, promotes tomato root growth. Therefore, the global transcriptome profiles of DADS-treated tomato roots over time were investigated to reveal the potential growth-promoting mechanisms. We detected 1828, 1296 and 1190 differentially expressed genes (DEGs) in the 4, 24 and 48 h samples, respectively. Most DEGs involved in assimilatory sulfate reduction and glutathione metabolism were up-regulated after short-term (4 h) DADS treatment. In addition, increased activity of defensive enzymes and up-regulation of six peroxidase genes were observed, suggesting that DADS could induce tomato resistance. In plant-pathogen interactions, DEGs related to calcium signaling were primarily inhibited, while those encoding pathogenesis-related proteins were primarily up-regulated. Although plant hormone synthesis and signal transduction were both significantly affected by DADS, the expression trends of the genes in these two pathways were conflicting. This research provides comprehensive information concerning the changes in the tomato root transcriptome affected by DADS and may help direct further studies on DADS-responsive genes to enhance the current understanding of the mechanisms by which DADS alleviates the obstacles to continuous cropping.