Moderate-Intensity Intermittent External Volume Expansion Optimizes the Soft-Tissue Response in a Murine Model
Moderate-Intensity Intermittent External Volume Expansion Optimizes the Soft-Tissue Response in a Murine Model
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DOI：
10.1097/prs.0000000000003190
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发表时间：
2017-04
影响因子：
3.6
通讯作者：
G. Giatsidis;Liying Cheng;F. Facchin;Anthony G Haddad;Jorge R. Lujan-Hernandez;L. Lancerotto;C. Nabzdyk;H. Matsumine;D. Orgill
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文献类型：
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作者：
G. Giatsidis;Liying Cheng;F. Facchin;Anthony G Haddad;Jorge R. Lujan-Hernandez;L. Lancerotto;C. Nabzdyk;H. Matsumine;D. Orgill
背景资料：使用抽吸进行间歇性外部容积扩张可增强软组织的血管网络，可能增加脂肪移植物的存活率。然而，最佳的应用动力学尚未确定。根据他们之前的经验，作者假设中等强度间歇性体外扩容应用可能进一步增强血管生成和脂肪生成潜力。研究方法：将50只12周龄野生型小鼠分配到5个实验组（每组n = 10），并进行5次不同的外部体积扩张的间歇性应用（即，单次应用对照、低强度、中等强度和两组高强度）。最后一次刺激后5天，从刺激区和对侧非刺激区获得皮肤活检标本。分析显微切片的血管生成、皮肤重塑和脂肪生成。结果如下：与对照组相比，中等强度间歇性刺激（0.5小时，6次/天，在-25 mmHg抽吸下持续5天）几乎使皮肤血管密度增加一倍（增加1.9倍），诱导皮肤增厚（增加1.9倍），并使皮下组织扩张（增加2.3倍）。在治疗后5天，外部体积扩张动力学不影响组织炎症。高强度间歇性刺激也增加了血管的密度（与对照组相比增加了1.6倍），但引起了组织损伤，而低强度的外部体积扩张并没有引起显着的变化。结论：应用中等强度间歇性外部容积扩张优化了软组织中血管生成和脂肪生成的诱导，而没有组织损伤，保持了脂肪移植前时间有效的原位预处理的潜力。
Background: Intermittent external volume expansion using suction enhances the vascular network of soft tissues, possibly increasing fat graft survival. However, the optimal kinetics of application have not been determined. Based on their previous experience, the authors hypothesized that moderate-intensity intermittent external volume expansion application may further enhance both the angiogenic and adipogenic potential. Methods: Fifty 12-week-old wild-type mice were assigned to five experimental groups (n = 10 per group) and underwent five different intermittent applications of external volume expansion (i.e., single-application control, low-intensity, moderate-intensity, and two groups of high-intensity). Five days after the final stimulation, skin biopsy specimens were obtained from stimulated and contralateral nonstimulated areas. Microscopic sections were analyzed for angiogenesis, skin remodeling, and adipogenesis. Results: Moderate-intensity intermittent stimulation (0.5 hour, 6 times/day for 5 days at −25 mmHg suction) almost doubled cutaneous vascular density (1.9-fold increase), induced skin thickening (1.9-fold increase), and expanded the subcutaneous tissue (2.3-fold increase) compared with control. External volume expansion kinetics did not affect tissue inflammation at 5 days after treatment. High-intensity intermittent stimulations also increased the density of blood vessels (1.6-fold increase compared with controls) but caused tissue damage, whereas low-intensity external volume expansion did not induce significant changes. Conclusion: Application of moderate-intensity intermittent external volume expansion optimizes induction of angiogenesis and adipogenesis in soft tissues without tissue damage, holding potential for time-effective recipient-site preconditioning before fat grafting.