INFECTIVITY OF RESPIRATORY SYNCYTIAL VIRUS BY VARIOUS ROUTES OF INOCULATION
INFECTIVITY OF RESPIRATORY SYNCYTIAL VIRUS BY VARIOUS ROUTES OF INOCULATION
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DOI：
10.1128/iai.33.3.779-783.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
GEIMAN, JM
中科院分区：
文献类型：
--
作者：
HALL, CB;DOUGLAS, RG;GEIMAN, JM
为了解呼吸道合胞病毒的传播情况，对不同途径接种不同剂量病毒的志愿者进行了感染频率的检测。成年志愿者(32人)接受了一种经过安全测试的呼吸道合胞病毒活毒株的系列稀释，注射到鼻子、眼睛或嘴里。最高接种量为5.2log1050%组织培养感染量(TCID50)，经鼻接种1组，经眼接种1组，经口接种2组，每组4人。随后，将1：100和1：1000稀释度的这种接种剂分别用鼻子和眼睛注射。在最高接种量时，经鼻接种的4名受试者中有3名感染，经眼睛接种的4名受试者中有3名感染。经口腔接种的8名受试者中有1人感染，但该受试者很可能是通过二次传播感染的。接种3.2log10 TCID50后，任何一种途径感染的受试者比例均降至25%。当接种量进一步减少到2.2log10 TCID50时，这两种途径都没有发生感染。在最高接种量后的感染以更早和更大的脱落为特征。呼吸道合胞病毒因此可能通过眼睛或鼻子感染，这两种途径似乎都同样敏感。相比之下，口腔似乎是一种不敏感的接种途径。这在感染控制程序和疫苗或其他预防措施的开发中具有潜在的重要意义。
To understand the transmission of respiratory syncytial virus, the frequency of infection in volunteers after inoculation by different routes with varying doses of virus was examined. Adult volunteers (32) received serial dilutions of a safety-tested live strain of respiratory syncytial virus instilled into nose, eye or mouth. The highest inoculum, 5.2 log10 50% tissue culture infective dose (TCID50), was administered to 4 groups of 4 subjects each, by nose to 1 group, by eye to 1 group and by mouth to 2 groups. Subsequently, 1:100 and 1:1000 dilutions of this inoculum were administered by nose and eye. At the highest inoculum, infection occurred in 3 of 4 subjects inoculated by nose and in 3 of 4 subjects inoculated by eye. Infection occurred in 1 of 8 subjects inoculated by mouth but this subject most likely was infected by secondary spread. With an inoculum of 3.2 log10 TCID50, the proportion of subjects infected by either route diminished to 25%. When the inoculum was further reduced to 2.2 log10 TCID50, no infections occurred by either route. Infections after the highest inoculum were characterized by earlier and greater shedding. Respiratory syncytial virus may thus infect by eye or nose and both routes appear equally sensitive. In comparison, the mouth appears to be an insensitive route of inoculation. This is of potential import in infection control procedures and in the development of vaccines or other prophylactic measures.