Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.
Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.
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DOI：
10.1038/s41467-021-21627-0
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发表时间：
2021-03-05
影响因子：
16.6
通讯作者：
Riesenberg S
中科院分区：
文献类型：
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作者：
Bokelmann L;Nickel O;Maricic T;Pääbo S;Meyer M;Borte S;Riesenberg S
遏制SARS-CoV-2传播的努力刺激了对可靠、快速和具有成本效益的诊断方法的需求，这些方法可以应用于大量人。然而，目前检测病毒核酸的标准方案虽然敏感，但需要高水平的自动化和先进的实验室设备才能实现产量，使整个社区能够定期进行检测。在这里，我们提出了Cap-iLamp(捕获和改进的环介导等温扩增)，它结合了基于杂交捕获的漱口液样本RNA提取与改进的比色RT-LAMP分析和基于智能手机的颜色评分。CAP-iLamp与护理点检测兼容，能够在不到一小时的时间内检测出SARS-CoV-2阳性样本。与直接将样品添加到改进的LAMP(ILamp)中不同，Cap-iLamp可防止假阳性，并允许在25个阴性样品池中检测单个阳性样品，从而将每次检测的试剂成本降低到每个人约1欧元。目前的SARS-CoV-2诊断方法很敏感，但不适合定期对整个社区进行检测。在这里，作者提出了Cap-iLamp，它用改进的比色RT灯测试漱口洗液样本。
Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual. Current SARS-CoV-2 diagnostic methods are sensitive yet poorly suited to testing whole communities on a regular basis. Here the authors present Cap-iLAMP that tests gargle lavage samples with an improved colorimetric RT-LAMP.