Using diet-induced obesity to understand a metabolic subtype of osteoarthritis in rats
Using diet-induced obesity to understand a metabolic subtype of osteoarthritis in rats
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DOI：
10.1016/j.joca.2015.01.015
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发表时间：
2015-06-01
影响因子：
7
通讯作者：
Herzog, W.
中科院分区：
文献类型：
--
作者：
Collins, K. H.;Reimer, R. A.;Herzog, W.
肥胖个体的骨关节炎（OA）通常归因于关节负荷。然而，OA的一个亚型，代谢性OA，可能是由于肥胖相关的内在因素，但仍有待实验评估对一个已知的OA progressionmodel.Objective：评估肥胖是否有助于OA发病使用高脂肪/高糖饮食诱导肥胖（DIO）模型与前交叉韧带横断大鼠（ACL-X）。Sprague道利大鼠（n = 33）食用高脂肪/高蔗糖或普通饲料12周，随机分为三组之一：单侧ACL-X组、假手术组或未经处理的非手术组。这些动物再随访16周。在牺牲，身体成分，膝关节改良的Mankin评分，和27血清和滑液细胞因子和adipokines measured.Results：实验肢体的肥胖ACL-X，肥胖假手术，瘦ACL-X动物有类似的改良的Mankin评分大于那些从瘦假手术和幼稚动物。肥胖对侧肢体的OA损伤与肥胖动物的ACL-X和假手术肢体以及瘦动物的ACL-X肢体相似。肥胖对侧肢体Modified Mankin评分与体脂百分比有较强的相关性（r = 0.75，P < 0.001）。血清瘦素和滑液IP 10/CXCL 10最好地描述了在对侧肢体的肥胖animals.Conclusions改良Mankin评分：机械因素在实验肢体OA损伤，如预期。有趣的是，肥胖对侧肢体的OA损伤与机械扰动肢体相似，表明肥胖可能以非机械方式诱导OA。(C)2015国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Osteoarthritis (OA) in obese individuals is often attributed to joint loading. However, a subtype of OA, Metabolic OA, may be due to obesity-related intrinsic factors but remains to be evaluated experimentally against a known OA progression model.Objective: To evaluate if obesity contributes to OA onset using a high fat/high sucrose diet-induced obesity (DIO) model with anterior cruciate ligament-transected rats (ACL-X).Methods: Sprague Dawley rats (n = 33) consumed high fat/high sucrose or chow diets for 12 weeks, were randomized to one of three groups: a unilateral ACL-X group, sham surgery group, or naive non-surgical group. These animals were followed for an additional 16 weeks. At sacrifice, body composition, knee joint Modified Mankin scores, and 27 serum and synovial fluid cytokines and adipokines were measured.Results: Experimental limbs of obese ACL-X, obese Sham, and lean ACL-X animals had similar Modified Mankin scores that were greater than those obtained from lean Sham and naive animals. Obese contralateral limbs had similar OA damage as ACL-X and Sham limbs of obese and ACL-X limbs of lean animals. Obese contralateral limb Modified Mankin scores had a strong correlation (r = 0.75, P < 0.001) with body fat percentage. Serum leptin and synovial fluid IP10/CXCL10 best described Modified Mankin scores in contralateral limbs of obese animals.Conclusions: Mechanical factors produced OA damage in experimental limbs, as expected. Interestingly, OA damage in obese contralateral limbs was similar to mechanically perturbed limbs, suggesting that obesity may induce OA in a non-mechanical manner. (C) 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.