Characterization of long-term endogenous cardiac repair in children after heart transplantation
Characterization of long-term endogenous cardiac repair in children after heart transplantation
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DOI：
10.1093/eurheartj/ehn223
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发表时间：
2008-08-01
影响因子：
39.3
通讯作者：
Dimmeler, Stefanie
中科院分区：
文献类型：
--
作者：
Rupp, Stefan;Koyanagi, Masamichi;Dimmeler, Stefanie
循环细胞在成年人中以非常低的速率重新填充心脏。关于时间依赖性心脏再生的知识非常有限，并且循环细胞对儿童心肌细胞或血管细胞的贡献是未知的。本研究探讨了内源性修复能力和长期纳入循环细胞在heart-transmitted children.Methods和Results的心脏和内皮嵌合体检测在9名儿童（年龄1个月-14岁）与性别不匹配的心脏移植荧光原位杂交的子宫内膜活检。从移植到活检的时间从1个月到10年不等。心肌细胞再生的程度为2.39 +/- 1.54%（范围：0-4.2%），与移植至活检取样的时间显著相关（r（2）= 0.69，P = 0.006; n = 9）。男性心肌细胞每年在女性心脏中的计算贡献为0.36 +/-0.09%。与之前的报道一致，血管细胞的掺入率高于心肌细胞（14.4 +/- 4.17%），但不存在时间依赖性相关性。结论循环细胞对儿童心脏移植后的心肌细胞和内皮细胞有贡献。心肌细胞再增殖的发生率以时间依赖性方式持续增加（类似于4% Y染色体（+）心肌细胞/10年），并且类似于在成人中观察到的心脏再生活性。
Aims Circulating cells repopulate the heart at a very low rate in adult humans. The knowledge about time-dependent cardiac regeneration is very limited and the contribution of circulating cells to cardiomyocytes or vascular cells in children is unknown. This study investigates the endogenous repair capacity and the long-term incorporation of circulating cells in heart-transplanted children.Methods and results Cardiac and endothelial chimerism was detected in endomyocardial biopsies of nine children (age 1 months-14 years) with sex-mismatched heart transplantation by fluorescence in situ hybridization. Time from transplantation to biopsy ranged from 1 month up to 10 years. The extent of repopulating cardiomyocytes was 2.39 +/- 1.54% (range: 0-4.2%) and correlated significantly with the time from transplantation to biopsy sampling (r(2) = 0.69, P = 0.006; n = 9). The calculated contribution of male cardiomyocytes in the female heart per year was 0.36 +/- 0.09%. Consistent with the previous reports, the incorporation of vascular cells was higher compared with cardiomyocytes (14.4 +/- 4.17%), but did not correlate in a time-dependent manner.Conclusion Circulating cells contribute to cardiomyocytes and endothelial cells in children after heart transplantation. The incidence of repopulating cardiomyocytes continuously increases in a time-dependent manner (similar to 4% Y- chromosome(+) cardiomyocytes/10 years) and resembles the cardiac regeneration activity observed in adults.