A female-biased expressed elongase involved in long-chain hydrocarbon biosynthesis and courtship behavior in Drosophila melanogaster
A female-biased expressed elongase involved in long-chain hydrocarbon biosynthesis and courtship behavior in Drosophila melanogaster
复制标题
DOI：
10.1073/pnas.0608142104
复制
发表时间：
2007-03-13
影响因子：
11.1
通讯作者：
Wicker-Thomas, Claude
中科院分区：
文献类型：
--
作者：
Chertemps, Thomas;Duportets, Line;Wicker-Thomas, Claude
黑腹果蝇产生性二态角质信息素，这是导致交配的求偶行为的关键成分。这些分子是碳氢化合物，男性的长度为23和25个碳(主要是一个双键)，女性的长度是27和29个碳(主要是两个双键)。在这里，我们描述了一个伸长酶基因Elof，它的表达倾向于女性。771个碱基的ORF编码一个257-aa的蛋白，与小鼠SSC1延伸酶的序列同源性最高(33%)。在酵母中表达的c DNA的活性是饱和脂肪酸和不饱和脂肪酸的伸长，直到C30。果蝇的RNAi基因敲除导致雌性碳氢化合物的戏剧性改变，C29二烯减少，C25二烯增加，并伴随着几个求偶参数的改变：交配潜伏期延长，交配尝试和交配次数减少。利用转化器基因的靶向表达使雄性的碳氢化合物图谱女性化，导致Elof的高表达水平，这表明该基因处于性别决定等级的控制之下。在仅合成C23和C25碳氢化合物的仿生果蝇中不表达Elof。这些结果有力地支持了Elof是黑腹潜蝇雌性信息素生物合成和求偶行为的关键酶的假说。
Drosophila melanogaster produces sexually dimorphic cuticular pheromones that are a key component of the courtship behavior leading to copulation. These molecules are hydrocarbons, with lengths of 23 and 25 carbons in males (mainly with one double bond) and 27 and 29 carbons in females (mainly with two double bonds). Here, we describe an elongase gene, eloF, with female-biased expression. The 771-bp ORF encodes a 257-aa protein that shows the highest sequence identity with mouse SSC1 elongase (33%). The activity of the cDNA expressed in yeast was elongation of saturated and unsaturated fatty acids up to C30. RNAi knockdown in Drosophila led to a dramatic modification of female hydrocarbons, with decreased C29 dienes and increased C25 dienes accompanied by a modification of several courtship parameters: an increase in copulation latency and a decrease in both copulation attempts and copulation. Feminization of the hydrocarbon profile in males by using targeted expression of the transformer gene resulted in high expression levels of eloF, suggesting that the gene is under the control of the sex-determination hierarchy. There is no expression of eloF in Drosophila simulans, which synthesize only C23 and C25 hydrocarbons. These results strongly support the hypothesis that eloF is a crucial enzyme for female pheromone biosynthesis and courtship behavior in D. melanogaster.