A single time-window for protein synthesis-dependent long-term memory formation after one-trial appetitive conditioning
A single time-window for protein synthesis-dependent long-term memory formation after one-trial appetitive conditioning
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DOI：
10.1111/j.1460-9568.2005.03970.x
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发表时间：
2005-03-01
影响因子：
3.4
通讯作者：
Benjamin, PR
中科院分区：
文献类型：
--
作者：
Fulton, D;Kemenes, I;Benjamin, PR
蛋白质合成通常被认为是长期记忆形成所必需的。通常有两个时期的敏感性阻断蛋白质合成已被描述，一个立即训练后，另一个几个小时后。我们希望将行为长期记忆（LTM）形成的蛋白质合成依赖性的时间与LTM记忆痕迹的电生理相关性联系起来。我们使用了蜗牛，因为一个试验食欲调节喂养使用化学条件刺激导致一个稳定的LTM痕迹，可以监测行为，然后电生理制剂从相同的动物。训练后10分钟注射茴香霉素（翻译抑制剂）阻断了行为LTM的形成。放线菌素D（转录抑制剂）也是有效的，在10分钟。当茴香霉素，在剂量被证明是有效的阻断中枢神经系统蛋白质的合成，注射训练后1，2，3，4，5和6小时，没有效果的回忆。这些结果表明，有一个单一的时间段的敏感性蛋白质合成抑制持续10分钟和1小时之间的训练后，没有证据表明第二个窗口的敏感性。一个电生理相关的LTM被发现是敏感的茴香霉素注射训练后10分钟。在LTM的详细时程研究中，仅发现一个蛋白质合成依赖期是不寻常的，这表明涉及蛋白质合成的巩固过程在一次试验食欲调节中相对较快，并在训练1小时内完成。
Protein synthesis is generally held to be essential for long-term memory formation. Often two periods of sensitivity to blockade of protein synthesis have been described, one immediately after training and another several hours later. We wished to relate the timing of protein synthesis-dependence of behavioural long-term memory (LTM) formation to an electrophysiological correlate of the LTM memory trace. We used the snail Lymnaea because one-trial appetitive conditioning of feeding using a chemical conditioned stimulus leads to a stable LTM trace that can be monitored behaviourally and then electrophysiologically in preparations made from the same animals. Anisomycin (an inhibitor of translation) injected 10 min after training blocked behavioural LTM formation. Actinomycin D (an inhibitor of transcription) was also effective at 10 min. When anisomycin, at doses shown to be effective in blocking central nervous system protein synthesis, was injected at 1, 2, 3, 4, 5 and 6 h after training there was no effect on recall. These results indicate that there is a single period of sensitivity to protein synthesis inhibition in Lymnaea lasting for between 10 min and 1 h after training with no evidence for a second window of sensitivity. An electrophysiological correlate of LTM was found to be sensitive to anisomycin injected 10 min after training. It is unusual to find only one period of protein synthesis-dependence in detailed time-course studies of LTM, and this suggests that the consolidation processes involving protein synthesis are relatively rapid in one-trial appetitive conditioning and complete within 1 h of training.