SAGE Profiling and demonstration of differential gene expression along the axial developmental gradient of lignifying xylem in loblolly pine (Pinus taeda)
SAGE Profiling and demonstration of differential gene expression along the axial developmental gradient of lignifying xylem in loblolly pine (Pinus taeda)
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DOI：
10.1093/treephys/22.5.301
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发表时间：
2002-04-01
期刊：
影响因子：
4
通讯作者：
Dean, JFD
中科院分区：
文献类型：
--
作者：
Lorenz, WW;Dean, JFD
木材形成已在细胞和生物化学水平上进行了广泛的研究，但对于基因表达和调控仍然知之甚少。作为鉴定木材形成相关基因和确定其在木材品质中作用的第一步，采用基因表达系列分析（SAGE）对10年生火炬松木质化木质部基因表达进行了定量分析。基于从树干的上部（冠部）或下部（基部）分离的木质化木质部产生两个SAGE文库。从冠木文库中分析了超过85，000个标签，代表最多27，398个表达基因，在基木文库中分析了超过65，000个标签，代表最多25，983个表达基因。结合这些数据集以反映在木质化木质部中表达的基因的总和，150，855个标签被编目，代表最多42，641个不同的基因。目前，这项研究代表了最广泛的分析，其在高等植物，并提供了一个定量描述的转录组代表的木质化木质部的10岁火炬松。
Wood formation has been studied extensively at the cellular and biochemical levels, but remains poorly understood with respect to gene expression and regulation. As a first step toward identifying genes specifically involved in wood formation and characterizing their roles in deter-mining wood quality, serial analysis of gene expression (SAGE) was used to quantify gene expression in lignifying xylem from a single, 10-year-old loblolly pine (Pinus taeda L.). Two SAGE libraries were generated based on lignifying xylem isolated from either the upper (crown) or lower (base) portions of the trunk. Over 85,000 tags representing a maximum of 27,398 expressed genes were analyzed from the crown wood library, and more than 65,000 tags, representing a maximum of 25,983 expressed genes, were analyzed in the base wood library. Combining these data sets to reflect the sum of genes expressed in lignifying xylem, 150,855 tags were cataloged, representing a maximum of 42,641 different genes. Currently, this study represents the most extensive analysis of its kind in a higher plant and provides a quantitative description of the transcriptome representing the lignifying xylem of a 10-year-old loblolly pine.