Bacteriophages Carrying Antibiotic Resistance Genes in Fecal Waste from Cattle, Pigs, and Poultry
Bacteriophages Carrying Antibiotic Resistance Genes in Fecal Waste from Cattle, Pigs, and Poultry
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DOI：
10.1128/aac.00535-11
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发表时间：
2011-10-01
影响因子：
4.9
通讯作者：
Muniesa, Maite
中科院分区：
文献类型：
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作者：
Colomer-Lluch, Marta;Imamovic, Lejla;Muniesa, Maite
这项研究评估了携带抗生素耐药基因的噬菌体在动物环境中的发生。bla（TEM）、bla（CTX-M）（簇1和9）和mecA通过定量PCR在来自猪、家禽和牛粪便废物的71个噬菌体DNA样品中定量。每毫升或每克样品检测到bla（TEM）的3至4 log（10）基因拷贝（GC）、bla（CTX-M）的2至3 log（10）GC和mecA的1至3 log（10）GC的密度，表明噬菌体可以是抗生素抗性基因水平转移的环境载体。
This study evaluates the occurrence of bacteriophages carrying antibiotic resistance genes in animal environments. bla(TEM), bla(CTX-M) (clusters 1 and 9), and mecA were quantified by quantitative PCR in 71 phage DNA samples from pigs, poultry, and cattle fecal wastes. Densities of 3 to 4 log(10) gene copies (GC) of bla(TEM), 2 to 3 log(10) GC of bla(CTX-M), and 1 to 3 log(10) GC of mecA per milliliter or gram of sample were detected, suggesting that bacteriophages can be environmental vectors for the horizontal transfer of antibiotic resistance genes.