Intracellular Uptake and Photodynamic Activity of Water-Soluble [60]- and [70]Fullerenes Incorporated in Liposomes
Intracellular Uptake and Photodynamic Activity of Water-Soluble [60]- and [70]Fullerenes Incorporated in Liposomes
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DOI：
10.1002/chem.200801090
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Nagasaki, Takeshi
中科院分区：
文献类型：
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作者：
Doi, Yuki;Ikeda, Atsushi;Nagasaki, Takeshi
水溶性富勒烯类化合物在光生物化学领域被用来开辟新的途径，引起了人们的注意。为了制备水溶性富勒烯，我们采用了脂膜包埋富勒烯(LMICx；，；x=60或70)的方法，通过从γ-环糊精(Gamma-CD)腔到囊泡的富勒烯交换方法。LMIC60在黑暗中毒性低，可通过光照射(波长350 nm)引起细胞死亡。在相同的光子通量(波长400 nm)下，LMIC70的光动力学活性是LMIC60的4.7倍。LMIC70的光毒性较高的原因之一是LMIC70比LMIC60产生更多的单线态氧(O-1(2))。LMIC60和LMIC70之间的差异被认为是由于适合光动力疗法(PDT)的400-700 nm区域的光吸收量而产生的。据我们所知，这是第一次测定CM及其衍生物对HeLa细胞的生物活性。
Water-soluble fullerenes have attracted attention compounds that have been used to forge new paths in the field of photobiochemistry. To prepare water-soluble fullerenes, we employed lipid-membrane-incorporated fullerenes (LMICx;,; x=60 or 70) by using the fullerene exchange method from a gamma-cyclodextrin (gamma-CD) cavity to vesicles. LMIC60 have low toxicity in the dark and engender cell death by photoirradiation (lambda> 350 nm). Furthermore, the photodynamic activity of LMIC70 is 4.7-fold that of LMIC60 for the same photon flux (lambda >400nm). One of the reasons for the higher phototoxicity of LMIC70 is the higher generation of singlet oxygen (O-1(2)) in LMIC70 than in LMIC60. The difference between LMIC60 and LMIC70 is considered to be simply derived from the amount of light absorption in the 400-700nm region that is suitable for photodynamic therapy (PDT). To the best of our knowledge, this is the first case in which biological activity Of CM and its derivatives toward HeLa cells has been assayed.