In vitro induction of tetraploidy in mulberry (Morus alba L.)
In vitro induction of tetraploidy in mulberry (Morus alba L.)
复制标题
DOI：
10.1007/s002990050486
复制
发表时间：
1998-07-01
影响因子：
6.2
通讯作者：
Qadri, SMH
中科院分区：
文献类型：
--
作者：
Chakraborti, SP;Vijayan, K;Qadri, SMH
桑树(Morus alba L.)在体外条件下进行顶芽处理。用三种不同浓度的秋水仙碱(0.05、0.1和0.2%)处理试管苗顶芽24 h，0.1%秋水仙碱处理组的四倍体率为39.4+/-4.8%，0.2%秋水仙碱处理组的四倍体率为16.7+/-2.3%。形态、组织学和细胞学证据表明，体外诱导的四倍体在表型和基因组上与体外诱导的四倍体相似。四倍体在含有2.6mU N-NAA的基本培养基上生根。四倍体的体外回收效率比体外回收高80.8%。用相同的秋水仙素培养基和附加的外植体培养4周，对四倍体的诱导是同样有效的。
A high frequency of tetraploidy was induced in mulbeny (Morus alba L.) through apical bud treatment under ia vitro conditions. Apical buds from in vitro-grown plants were treated with three different concentrations (0.05, 0.1 and 0.2%) of colchicine in MS medium for 24 h. Tetraploidy at a frequency of 39.4+/-4.8% was obtained using 0.1% colchicine, whereas the frequency of tetraploidy was significantly reduced to 16.7+/-2.3% when 0.2% colchicine was used. Morphological, histological and cytological evidence indicated a phenotypic and genomic similarity of in vitro- with ex vitro-induced tetraploids. Rooting of tetraploids was on basal medium containing 2.6 mu M NAA. The recovery of tetraploids was 80.8% more efficient using the in vitro method instead of the ex vitro method. The use of the same colchicine medium for up to 4 weeks with additional explants was found to be equally effective for the induction of tetraploidy.