MiR-140-3p suppressed cell growth and invasion by downregulating the expression of ATP8A1 in non-small cell lung cancer
MiR-140-3p suppressed cell growth and invasion by downregulating the expression of ATP8A1 in non-small cell lung cancer
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DOI：
10.1007/s13277-015-3452-9
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发表时间：
2016-03-01
期刊：
影响因子：
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通讯作者：
Li, Baosheng
中科院分区：
文献类型：
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作者：
Dong, Wei;Yao, Chunping;Li, Baosheng
microRNAs（miRNAs）是一类非编码小分子RNA，调控靶基因的表达。据报道，microRNA的失调参与癌发生和肿瘤进展。在这里，我们确定了miR-140- 3 p作为一个下调的microRNA在大多数癌症组织，包括肺癌组织，与他们的正常同行。miR-140- 3 p通过抑制细胞生长、迁移和侵袭而诱导细胞凋亡来发挥其抑癌作用。此外，miR-140- 3 p的过表达抑制了裸鼠模型中非小细胞肺癌（NSCLC）细胞的生长。使用荧光素酶测定证明ATP 8A 1是miR-140- 3 p的新的直接靶点。细胞内ATP 8A 1蛋白表达水平的增加减弱了miR-140- 3 p对NSCLC细胞生长和迁移的抑制作用。本研究首次发现miR-140- 3 p通过下调ATP 8A 1的表达调控NSCLC的发生发展。
MicroRNAs (miRNAs) as a class of small noncoding RNA molecules regulate the expression of targeted gene. The dysregulation of microRNAs is reported to be involved in carcinogenesis and tumor progression. Here, we identified miR-140-3p as a downregulated microRNA in most cancer tissues including lung cancer tissues, compared with their normal counterparts. MiR-140-3p was observed to perform its tumor suppressor function via its inhibition on cell growth, migration and invasion but its induction of cell apoptosis. Furthermore, the growth of non-small-cell lung cancer (NSCLC) cells in nude mouse models were suppressed by overexpression of miR-140-3p. ATP8A1 was demonstrated as a novel direct target of miR-140-3p using a luciferase assay. The increased level of intracellular ATP8A1 protein attenuated the inhibitor role of miR-140-3p in the growth and mobility of NSCLC cell. A regulation mechanism of miR-140-3p for the development and progression of NSCLC through downregulating the ATP8A1 expression was first discovered in the present study.