In vitro characteristics of rat mesangial cells in comparison with aortic smooth muscle cells and dermal fibroblasts
In vitro characteristics of rat mesangial cells in comparison with aortic smooth muscle cells and dermal fibroblasts
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大鼠系膜细胞与主动脉平滑肌细胞和真皮成纤维细胞的体外特征比较
DOI：
10.1007/bf02912106
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
I. Kihara
中科院分区：
文献类型：
--
作者：
Eishin Yaoita;T. Kazama;K. Kawasaki;S. Miyazaki;Tadashi Yamamoto;I. Kihara
本实验培养了大鼠肾小球系膜细胞，并将其抗原与主动脉血管平滑肌细胞和真皮成纤维细胞的抗原进行了比较。将肾小球、主动脉和真皮澄清剂培养3周，并在相同条件下传代培养。使用抗Thy-1抗原、结蛋白和鸡砂囊肌动蛋白的抗体通过间接免疫荧光研究评价这些培养的细胞。肾小球系膜细胞Thy-1、结蛋白、肌动蛋白阳性。另一方面，成纤维细胞结蛋白阴性，平滑肌细胞几乎不染色Thy-1，结蛋白阴性。在后两种细胞中，肌动蛋白阳性纤维比系膜细胞更细、更暗。这些结果表明，免疫荧光显微镜可以在体外将系膜细胞与血管平滑肌细胞和成纤维细胞区分开来。
SummaryRat glomerular mesangial cells were cultured and their antigens were compared with those of aortic vascular smooth muscle cells and dermal fibroblasts. Glomeruli, aortic, and dermal expiants were cultured for 3 weeks and subcultured in the same conditions. These cultured cells were evaluated by indirect immunofluorescence studies using antibodies against Thy-1 antigen, desmin, and chicken gizzard actin. Most of mesangial cells were positive for Thy-1, desmin, and actin. On the other hand, fibroblasts were negative for desmin, and smooth muscle cells stained Thy-1 scarcely, and were negative for desmin. In the latter two cells, actin-positive fibrils were thinner and fainter than mesangial cells. These results indicated that mesangial cells could be distinguished in vitro from vascular smooth cells and fibroblasts by immunofluorescence microscopy.
体外人肾小球细胞：分离和表征。
影响因子：
0.9
作者：
Striker,GE;Killen,PD;Farin,FM
通讯作者：
Farin,FM