Leaf Rolling Controlled by the Homeodomain Leucine Zipper Class IV Gene Roc5 in Rice
Leaf Rolling Controlled by the Homeodomain Leucine Zipper Class IV Gene Roc5 in Rice
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水稻中同源域亮氨酸拉链 IV 类基因 Roc5 控制叶片卷曲
DOI：
10.1104/pp.111.176016
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发表时间：
2011-07-01
期刊：
影响因子：
7.4
通讯作者：
Lu, Tie-gang
中科院分区：
文献类型：
--
作者：
Zou, Liang-ping;Sun, Xue-hui;Lu, Tie-gang
卷叶是水稻育种中一项重要的农艺性状。为了了解控制叶片滚动的分子机制，我们筛选了一个水稻T-DNA插入群体，并分离出具有叶片背面滚动的外曲叶1 （outcurved leaf1, oul1）突变体。表型是通过敲除水稻最外层细胞特异性基因5 （Roc5）引起的，该基因是拟南芥（Arabidopsis thaliana）同源结构域亮氨酸拉链IV类基因GLABRA2的同源基因。有趣的是，过表达Roc5导致叶片向正面滚动，而共抑制Roc5导致叶片向反面滚动。在oul1和Roc5共抑制植株中，球状细胞数量和大小增加，而在Roc5过表达系中，球状细胞数量和大小减少。数据表明，Roc5负调控球状细胞的命运和发育。基因表达谱、定量聚合酶链反应和RNA干扰（RNAi）分析显示，原真皮因子样（PFL）可能在oul1中下调。在过表达Roc5的细胞系中，PFL mRNA水平升高，并且由于球状细胞数量和大小的增加，PFL- rnai转基因植株表现出叶片的反向滚动，表明Roc5可能具有保守功能。据我们所知，这是水稻中编码同域亮氨酸拉链IV类转录因子的基因的第一个功能数据，该基因调节叶片滚动。
Leaf rolling is considered an important agronomic trait in rice (Oryza sativa) breeding. To understand the molecular mechanism controlling leaf rolling, we screened a rice T-DNA insertion population and isolated the outcurved leaf1 (oul1) mutant showing abaxial leaf rolling. The phenotypes were caused by knockout of Rice outermost cell-specific gene5 (Roc5), an ortholog of the Arabidopsis (Arabidopsis thaliana) homeodomain leucine zipper class IV gene GLABRA2. Interestingly, overexpression of Roc5 led to adaxially rolled leaves, whereas cosuppression of Roc5 resulted in abaxial leaf rolling. Bulliform cell number and size increased in oul1 and Roc5 cosuppression plants but were reduced in Roc5-overexpressing lines. The data indicate that Roc5 negatively regulates bulliform cell fate and development. Gene expression profiling, quantitative polymerase chain reaction, and RNA interference (RNAi) analyses revealed that Protodermal Factor Like (PFL) was probably down-regulated in oul1. The mRNA level of PFL was increased in Roc5-overexpressing lines, and PFL-RNAi transgenic plants exhibit reversely rolling leaves by reason of increases of bulliform cell number and size, indicating that Roc5 may have a conserved function. These are, to our knowledge, the first functional data for a gene encoding a homeodomain leucine zipper class IV transcriptional factor in rice that modulates leaf rolling.