The reduced folate carrier in L1210 murine leukemia cells is a 58-kDa protein.
The reduced folate carrier in L1210 murine leukemia cells is a 58-kDa protein.
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L1210 鼠白血病细胞中的还原叶酸载体是一种 58 kDa 的蛋白质。
DOI：
10.1016/s0005-2736(00)00190-5
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Goldman,ID
中科院分区：
文献类型：
--
作者：
Zhao,R;Gao,F;Liu,L;Goldman,ID
还原叶酸载体（RFC 1）是天然还原叶酸和抗叶酸化疗药物的主要转运蛋白。使用针对第六和第七预测跨膜结构域之间的环或远端C-末端的表位的多克隆抗体，我们能够通过Western印迹分析证明，在小鼠白血病L1210细胞中表达的RFC 1的分子大小为58 kDa，如其cDNA的开放阅读框所预测的。46-仅在质膜制备物中检测到的38-kDa蛋白是在膜制备或用常规SDS-PAGE加载缓冲液处理期间出现的蛋白水解降解产物。这些数据解决了先前报道的RFC 1分子大小的差异。
The reduced folate carrier (RFC1) is a major transporter for both natural reduced folates and antifolate chemotherapeutics. Using polyclonal antibodies targeted to epitopes at the loop between the sixth and seventh predicted transmembrane domains or the distal C-terminus, we were able to demonstrate by Western blot analysis that the molecular size of RFC1 expressed in murine leukemia L1210 cells is 58 kDa as predicted by the open reading frame of its cDNA. 46- and 38-kDa proteins detected only in plasma membrane preparations were proteolytic degradation products that appeared during membrane preparation or treatment with the conventional SDS–PAGE loading buffer. These data resolve discrepancies reported previously for the molecular size of RFC1.