Cryptococcus laurentii controls gray mold of cherry tomato fruit via modulation of ethylene-associated immune responses
Cryptococcus laurentii controls gray mold of cherry tomato fruit via modulation of ethylene-associated immune responses
复制标题
劳伦隐球菌通过调节乙烯相关的免疫反应来控制樱桃番茄果实的灰霉病
DOI：
10.1016/j.foodchem.2018.11.051
复制
发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Lu Laifeng
中科院分区：
文献类型：
--
作者：
Tang Qiong;Zhu Fanghuan;Cao Xuan;Zheng Xiaodong;Yu Ting;Lu Laifeng
本研究旨在探讨植物激素乙烯在樱桃番茄果实抗灰霉病免疫反应中的作用。用拮抗酵母菌罗伦隐球菌预处理可显著降低B. cinereaininfection，并伴随着乙烯的生产在整个水果的爆发。用1-MCP（5 μL L-1或更高）阻断乙烯的感受，显著削弱了果实自身的保护能力，抑制了C.劳伦氏菌刺激的宿主免疫应答。5 μL L−11-MCP预熏处理降低了乙烯生物合成和感知相关基因SlACO 1、SlACS 2、SlERF 1、SlPti 5和SlMPK 3的表达，降低了C.劳伦特处理的水果。结果表明，1-MCP预熏处理对酵母菌表达上调的SlCHI 9、SlGlub、SlPAL 3、SlPR 1和SlPR 5基因的表达均有不同程度的抑制作用。这些结果表明乙烯有助于水果免疫和C. laurentii介导的樱桃番茄的免疫应答。
This research aimed to investigate the roles of phytohormone ethylene in cherry tomato fruit immune response against gray mold caused byBotrytis cinerea. Pretreatment with antagonistic yeastCryptococcus laurentiiresulted in a significantly decreased disease incidence ofB. cinereainfection, and accompanied by a burst of ethylene production in the whole fruit. Blocking the ethylene perception by adding 1-MCP (5 μL L−1or greater) remarkably weaken the protection ability of fruit itself and suppressed theC. laurentii-stimulated host immune response. 5 μL L−11-MCP prefumigation decreased the expression of ethylene biosynthesis and perception related genesSlACO1,SlACS2,SlERF1,SlPti5andSlMPK3, and ethylene production inC. laurentiitreated fruit. Consequently, the expressions ofSlCHI9,SlGlub,SlPAL3,SlPR1andSlPR5up-regulated by the yeast were all impaired to different degrees by the 1-MCP prefumigation. These findings demonstrate that ethylene contributes to fruit immunity andC. laurentii-mediated immune responses of cherry tomato.