Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in Balanus improvisus cyprid settlement.
Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in Balanus improvisus cyprid settlement.
复制标题
研究细菌信号分子 N-酰基高丝氨酸内酯在龟头龟定居中的可能作用。
DOI：
10.1111/mec.12273
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发表时间：
2013
影响因子：
4.9
通讯作者：
Tait K
中科院分区：
文献类型：
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作者：
Tait K
一些海洋无脊椎动物幼虫在细菌生物膜上的沉着增加已经被证明，但线索的性质(S)负责还不太清楚。我们检验了海湾藤壶利用细菌信号分子N-酰基高丝氨酸内酯(AHLS)作为选择永久附着地点的线索的假设。产AHL细菌鳗弧菌、嗜水气单胞菌和硫杆菌的单种生物被膜BR_1对定居鲤鱼幼虫有较强的引诱作用。然而，当AHL合成基因的突变或AHL降解基因(AIIA)的表达使AHL产生失活时，细菌吸引鲤鱼的能力就会消失。此外，鲤鱼还积极探索表达费氏弧菌重组AHL合成酶基因流I(3-oxo-C6-HSL)、铜绿假单胞菌重组AHL合成酶基因流(C4-HSL/C6-HSL)、红色弧菌VIfrom ganguillarum(3-oxo-C10-HSL)和硫酸盐杆菌SulI的生物膜。BR1(C4-HSL，3-羟基-C6-HSL，C8-HSL和3-羟基-C10-HSL)，但注意不产生AHL的大肠埃希菌。最后，合成AHL(C8-HSL、3-OXO-C10-HSL和C12-HSL)的浓度与天然生物膜中发现的浓度(5μm)相似，导致鲤虫沉降量增加。因此，B.cypris对生物膜的即兴探索和在生物膜上的定居似乎是由实验室中的AHL信号细菌介导的。这增加了我们对群体感应抑制如何用于生物污染控制的理解。尽管如此，我们的结果对于幼虫在野外自然定居的意义，以及观察到的对AHL反应的机制，目前尚不清楚。
Increased settlement on bacterial biofilms has been demonstrated for a number of marine invertebrate larvae, but the nature of the cue(s) responsible is not well understood. We tested the hypothesis that the bay barnacleBalanus improvisusutilizes the bacterial signal molecules N‐acylhomoserine lactones (AHLs) as a cue for the selection of sites for permanent attachment. Single species biofilms of the AHL‐producing bacteriaVibrio anguillarum,Aeromonas hydrophilaandSulfitobactersp. BR1 were attractive to settling cypris larvae ofB. improvisus. However, when AHL production was inactivated, either by mutation of the AHL synthetic genes or by expression of an AHL‐degrading gene (aiiA), the ability of the bacteria to attract cyprids was abolished. In addition, cyprids actively explored biofilms ofE. coliexpressing the recombinant AHL synthase genesluxIfromVibrio fischeri(3‐oxo‐C6‐HSL),rhlIfromPseudomonas aeruginosa(C4‐HSL/C6‐HSL),vanIfromV. anguillarum(3‐oxo‐C10‐HSL) andsulIfromSulfitobactersp. BR1 (C4‐HSL, 3‐hydroxy‐C6‐HSL, C8‐HSL and 3‐hydroxy‐C10‐HSL), but notE. colithat did not produce AHLs. Finally, synthetic AHLs (C8‐HSL, 3‐oxo‐C10‐HSL and C12‐HSL) at concentrations similar to those found within natural biofilms (5 μm) resulted in increased cyprid settlement. Thus,B. improvisuscypris exploration of and settlement on biofilms appears to be mediated by AHL‐signalling bacteria in the laboratory. This adds to our understanding of how quorum sensing inhibition may be used as for biofouling control. Nonetheless, the significance of our results for larvae settling naturally in the field, and the mechanisms that underlay the observed responses to AHLs, is as yet unknown.