Chemical Protein Synthesis by Kinetically Controlled Ligation of Peptide O‐Esters
Chemical Protein Synthesis by Kinetically Controlled Ligation of Peptide O‐Esters
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DOI：
10.1002/cbic.200900789
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发表时间：
2010-03
期刊：
影响因子：
3.2
通讯作者：
Ji‐Shen Zheng;Hong‐Kui Cui;Gemin Fang;Weixian Xi;Lei Liu
中科院分区：
文献类型：
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作者：
Ji‐Shen Zheng;Hong‐Kui Cui;Gemin Fang;Weixian Xi;Lei Liu
蛋白质化学合成可以克服蛋白质表达的潜在限制，并以原子精度生产具有预先设计的变化和修饰的蛋白质。开发日益高效和通用的肽连接方法是一个中心目标。一项重要的挑战是通过顺序连接三个或更多未受保护的肽片段来合成蛋白质。早期的策略侧重于从 C 末端 Cys 肽片段到 N 末端的顺序连接，但肽片段的相应 N 到 C 顺序组装很困难，这阻碍了完全收敛的蛋白质合成。为了解决这个问题，肯特等人。最近发明了动力学控制连接（KCL；方案1）。 KCL的成功依赖于大
Protein chemical synthesis can overcome potential limitations of protein expression and produce proteins with predesigned changes and modifications with atomic precision. The development of increasingly efficient and general methods for peptide ligation comprises a central objective. One important challenge is to synthesize proteins by sequential ligation of three or more unprotected peptide segments. Earlier strategies focused on sequential ligations toward the N terminus from a Cterminal Cys peptide segment, but the counterpart N-to-C sequential assembly of the peptide segments was difficult, and this prevented fully convergent protein synthesis. To solve the problem Kent et al. recently invented kinetically controlled ligation (KCL; Scheme 1). The success of KCL relies on the large