A new generation of Ca2+ indicators with greatly improved fluorescence properties.
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
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发表时间：
1985-03
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通讯作者：
G. Grynkiewicz;M. Poenie;Roger Y. TsienB
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作者：
G. Grynkiewicz;M. Poenie;Roger Y. TsienB
合成了一个新的高荧光指示剂家族，用于细胞溶质游离Ca 2+生理作用的生化研究。该化合物联合收割机将8-配位四羧酸螯合位点与二苯乙烯发色团结合。将二苯乙烯的烯键结合到杂环中提高了荧光团的量子效率和光化学稳定性。与其广泛使用的前身“quin 2”相比，新染料提供高达30倍的更亮荧光，在Ca 2+结合时波长的主要变化不仅仅是强度，对Ca 2+的亲和力略低，激发波长略长，并且对Ca 2+的选择性大大提高。这些性质，特别是对Ca 2+的波长敏感性，应该使这些染料成为许多细胞内应用的优选荧光指示剂，特别是在单细胞、粘附细胞层或大块组织中。
A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+. The compounds combine an 8-coordinate tetracarboxylate chelating site with stilbene chromophores. Incorporation of the ethylenic linkage of the stilbene into a heterocyclic ring enhances the quantum efficiency and photochemical stability of the fluorophore. Compared to their widely used predecessor, "quin2", the new dyes offer up to 30-fold brighter fluorescence, major changes in wavelength not just intensity upon Ca2+ binding, slightly lower affinities for Ca2+, slightly longer wavelengths of excitation, and considerably improved selectivity for Ca2+ over other divalent cations. These properties, particularly the wavelength sensitivity to Ca2+, should make these dyes the preferred fluorescent indicators for many intracellular applications, especially in single cells, adherent cell layers, or bulk tissues.