Behavioral and physiological responses to intraperitoneal injection of zymosan in chicks
Behavioral and physiological responses to intraperitoneal injection of zymosan in chicks
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DOI：
10.1016/j.domaniend.2020.106471
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发表时间：
2020-07-01
影响因子：
2.1
通讯作者：
Cline, M. A.
中科院分区：
文献类型：
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作者：
Tachibana, T.;Nakatani, A.;Cline, M. A.
酵母聚糖是酿酒酵母的细胞壁成分，在哺乳动物中产生严重的炎症反应。当酵母多糖被注射到哺乳动物的外周时，它会引起一些行为和生理变化，包括厌食和体温过高。然而，据我们所知，行为和生理反应的酵母多糖还没有明确的鸟类。因此，本研究的目的是确定腹腔注射酵母多糖是否影响鸡（原鸡）的摄食量，自主活动，泄殖腔温度，血浆皮质酮（CORT）和葡萄糖浓度，以及脾脏细胞因子的基因表达。腹腔注射酵母多糖（2.5毫克）显着减少食物摄入量，自主活动，血糖浓度，并增加血浆CORT浓度。最后，2.5mg酵母多糖显著增加脾脏白细胞介素-1b（IL-1b）、IL-6、IL-8、干扰素-γ和肿瘤坏死因子样细胞因子1A的基因表达。本研究结果提示酵母多糖可能是真菌感染后引起雏鸡厌食、活动减少、体温升高和应激反应等非特异性症状的成分之一。(C)2020爱思唯尔公司All rights reserved.
Zymosan is a cell wall component of the yeast Saccharomyces cerevisiae and produces severe inflammatory responses in mammals. When zymosan is peripherally injected in mammals, it induces several behavioral and physiological changes including anorexia and hyperthermia. However, to our knowledge, behavioral and physiological responses to zymosan have not yet been clarified in birds. Therefore, the purpose of the present study was to determine if intraperitoneal injection of zymosan affects food intake, voluntary activity, cloacal temperature, plasma corticosterone (CORT) and glucose concentrations, and splenic gene expression of cytokines in chicks (Gallus gallus). Intraperitoneal injection of zymosan (2.5 mg) significantly decreased food intake, voluntary activity, and plasma glucose concentration, and increased plasma CORT concentration. The injection of 0.5 mg zymosan significantly increased cloacal temperature, while 2.5 mg zymosan had a tendency to increase it. Finally, 2.5 mg zymosan significantly increased the splenic gene expression of interleukin-1b (IL-1b), IL-6, IL-8, interferon-gamma, and tumor necrosis factor-like cytokine 1A. The present results suggest that zymosan would be one of components which induces nonspecific symptoms including anorexia, hypoactivity, hyperthermia, and stress responses, under fungus infection in chicks. (C) 2020 Elsevier Inc. All rights reserved.