EFFECT OF TEMPERATURE ON MACROPHAGE ACTIVATION AND THE PRODUCTION OF MACROPHAGE ACTIVATING FACTOR BY RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) LEUKOCYTES
EFFECT OF TEMPERATURE ON MACROPHAGE ACTIVATION AND THE PRODUCTION OF MACROPHAGE ACTIVATING FACTOR BY RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) LEUKOCYTES
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DOI：
10.1016/0145-305x(94)90252-6
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发表时间：
1994-01-01
影响因子：
2.9
通讯作者：
SECOMBES, CJ
中科院分区：
文献类型：
--
作者：
HARDIE, LJ;FLETCHER, TC;SECOMBES, CJ
巨噬细胞活化因子（MAF）的虹鳟鱼白细胞的生产已被证明是在体内和体外的温度依赖性。来自保持在14摄氏度并在分离后立即刺激以产生MAF的鱼的细胞能够在低至6摄氏度（测试的最低温度）下分泌MAF。然而，在6 ° C下48小时后，这些白细胞显示出受损的MAF分泌。鱼适应低温（7摄氏度）没有恢复低的体外温度对MAF生产的抑制作用，但如果这些白细胞在10或18摄氏度预孵育48小时，MAF从这些细胞产生。有趣的是，从保持在7或14摄氏度并在低温（6摄氏度）下培养的鱼中分离的巨噬细胞对含MAF的上清液有反应，并且与在10和18摄氏度下培养的对应物相比，其呼吸爆发活性显示出更高的相对增加。这样的观察清楚地表明，在低温下杀菌活性的主要损害存在于鱼的特定免疫区室中。对鱼类健康的影响进行了讨论。
Production of macrophage activating factor (MAF) by rainbow trout leucocytes has been shown to be temperature dependent in vivo and in vitro. Cells from fish held at 14 degrees C and stimulated to produce MAF immediately after isolation were capable of secreting MAF down to 6 degrees C (the lowest temperature tested). However, after 48 h at 6 degrees C, these leucocytes show impaired MAF secretion. Acclimation of fish to low temperatures (7 degrees C) did not recover the inhibitory effects of low in vitro temperatures on MAF production, but if these leucocytes were preincubated at 10 or 18 degrees C for 48 h, MAF was produced from these cells. Interestingly, macrophages isolated from fish kept at 7 or 14 degrees C and cultured at low temperatures (6 degrees C) were responsive to MAF-containing supernatants, and showed a higher relative increase in respiratory burst activity compared with their counterparts cultured at 10 and 18 degrees C. Such observations clearly demonstrate that a major impairment of bactericidal activity at low temperatures resides within the specific immune compartment of fish. The implications for fish health are discussed.