ETR1/RDO3 Regulates Seed Dormancy by Relieving the Inhibitory Effect of the ERF12-TPL Complex on DELAY OF GERMINATION1 Expression
ETR1/RDO3 Regulates Seed Dormancy by Relieving the Inhibitory Effect of the ERF12-TPL Complex on DELAY OF GERMINATION1 Expression
复制标题
ETR1/RDO3 通过解除 ERF12-TPL 复合物对 GERMINATION1 表达延迟的抑制作用来调节种子休眠
DOI：
10.1105/tpc.18.00449
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发表时间：
2019-04-01
期刊：
影响因子：
11.6
通讯作者：
Liu, Yongxiu
中科院分区：
文献类型：
--
作者：
Li, Xiaoying;Chen, Tiantian;Liu, Yongxiu
乙烯对种子休眠的控制已经得到了很好的研究，但其潜在的分子机制还不完全清楚。在这里，我们报道了拟南芥突变体减少休眠3(Rdo3)的特征及其相关基因的克隆。我们证明rdo3是乙烯受体乙烯反应1(ETR1)的功能缺失突变体。ETR1部分通过延缓GERMINATION1(DOG1)途径控制种子休眠。分子和遗传分析表明，乙烯反应因子12(ERF12)参与了ETR1下游种子休眠的调节。ERF12与无上装(TPL)相互作用，在基因上需要TPL发挥作用。ERF12和TPL通过占据Dog1启动子来抑制Dog1的表达。因此，我们确定了休眠途径ETR1-ERF12-TPL-DOG1，并通过将乙烯途径和DOG1途径联系起来，为调节种子休眠提供了机械性的见解。
The control of seed dormancy by ethylene has been well studied, but the underlying molecular mechanisms are not fully understood. Here, we report the characterization of the Arabidopsis (Arabidopsis thaliana) mutant reduced dormancy 3 (rdo3) and the cloning of the underlying gene. We demonstrate that rdo3 is a loss-of-function mutant of the ethylene receptor ETHYLENE RESPONSE1 (ETR1). ETR1 controls seed dormancy partially through the DELAY OF GERMINATION1 (DOG1) pathway. Molecular and genetic analyses demonstrated that ETHYLENE RESPONSE FACTOR12 (ERF12) is involved in the regulation of seed dormancy downstream of ETR1. ERF12 interacts with TOPLESS (TPL) and genetically requires TPL to function. ERF12 and TPL repress the expression of DOG1 by occupying its promoter. Thus, we identified the dormancy pathway ETR1-ERF12-TPL-DOG1 and provide mechanistic insights into the regulation of seed dormancy by linking the ethylene and DOG1 pathways.