Defining wild-type life span in Caenorhabditis elegans
Defining wild-type life span in Caenorhabditis elegans
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DOI：
10.1093/gerona/55.5.b215
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发表时间：
2000-05-01
影响因子：
5.1
通讯作者：
Riddle, DL
中科院分区：
文献类型：
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作者：
Gems, D;Riddle, DL
线虫秀丽隐杆线虫主要以自体雌雄同体的形式繁殖，这使得实验室种群在所有遗传位点上都是纯合的。原种的传代可导致自发突变的固定，特别是当后者在实验室条件下不导致选择性劣势时。寿命可能与一个性状有关，因为对来自共同祖先的6个野生型N2品系（但在几个实验室中分别保存）的比较显示，在20 ℃下，4个变体的中位成年寿命范围为12.0 +/- 0.8至17.0 +/- 0.6天。最短命的tao品种的生育能力也降低了。我们通过两种方法确定哪种寿命最接近于真实野生型的寿命。首先，将N2两性体与7个C.秀丽隐杆线虫野生分离株后者被发现只类似于寿命最长的N2菌株。雄性寿命的sis线的比较也揭示了额外的应变变化。其次，从N2变体之间的杂交产生的F1后代的寿命表明，短寿命是隐性的，这表明它们是由功能丧失突变引起的。我们推断，寿命最长的N2变种最像原来的N2分离。这是N2雄性股票目前分发的小杆线虫遗传学中心。
The nematode Caenorhabditis elegans reproduces predominantly as a self-fertilizing hermaphrodite, and this drives laboratory populations to be homozygous at all genetic loci. Passaging of stocks can lead to fixation of spontaneous mutations, especially when the latter do not result in a selective disadvantage under laboratory conditions. Life span may be with a trait, since a comparison of six wild-type N2 Lines derived from a common ancestor (but maintained separately in several laboratories) revealed four variants with median adult life spans ranging from 12.0 +/- 0.8 to 17.0 +/- 0.6 days at 20 degrees C. Fertility was also reduced in the tao shortest-lived strains. We determined which life span most closely corresponds to that of the authentic wild type by two means. Firstly, N2 hermaphrodites were compared with seven C. elegans wild isolates. The latter were found to resemble only the longest-lived N2 strain. Comparison of male life spans of sis lines also revealed additional strain variation. Secondly, life spans of F1 progeny issuing from crosses between N2 variants showed that short life spans were recessive, indicating that they result from loss-of-function mutations. We infer that the longest-lived N2 variant best resembles the original N2 isolate. This is the N2 male stock currently distributed by the Caenorhabditis Genetics Center.